Walther Hendrik, Subbaraman Lakshman N, Jones Lyndon
Centre for Ocular Research & Education (CORE-formerly known as CCLR), School of Optometry and Vision Science, University of Waterloo, Waterloo, ON, Canada.
Eye Contact Lens. 2019 Mar;45(2):105-111. doi: 10.1097/ICL.0000000000000547.
To determine the efficacy of multipurpose solutions (MPSs) on the removal of cholesterol deposits from silicone hydrogel (SH) contact lens materials using an in vitro model.
Five SH lens materials: senofilcon A, comfilcon A, balafilcon A, lotrafilcon A, and lotrafilcon B were removed from the blister pack (n=4 for each lens type), incubated for 7 days at 37°C in an artificial tear solution containing C radiolabeled cholesterol. Thereafter, lenses were stored in a preserved saline solution control (Sensitive Eyes Saline Plus) or cleaned with 1 of the 5 MPSs incorporating different preservatives (POLYQUAD/ALDOX, polyquaternium-1/alexidine, polyquaternium-1/PHMB, and 2 based on PHMB alone) using a rub and rinse technique, according to the manufacturer's recommendations, and stored in the MPS for a minimum of 6 hr. Lenses were then extracted with 2:1 chloroform:methanol, analyzed in a beta counter, and μg/lens of cholesterol was determined.
Balafilcon A and senofilcon A lens materials showed the highest amounts of accumulated cholesterol (0.93±0.02 μg/lens; 0.95±0.01 μg/lens, respectively), whereas lotrafilcon A and lotrafilcon B deposited the lowest amounts (0.37±0.03 μg/lens; 0.47±0.12 μg/lens, respectively). For all lens materials, the MPS preserved with POLYQUAD/ALDOX removed more deposited cholesterol than any other test solution; however, the amount of removed cholesterol contamination from the individual contact lenses was only statistically significant for balafilcon A and senofilcon A (P=0.006 and P=0.042, respectively). Sensitive eyes and the other evaluated MPSs showed no significant effect on cholesterol removal (P>0.05).
Cholesterol-removal efficacy varies depending on the combination of lens material and solution. Only 1 MPS showed a statistically significant reduction of cholesterol deposit for only 2 of the 5 tested lens materials.
使用体外模型确定多用途护理液（MPS）对去除硅水凝胶（SH）隐形眼镜材料上胆固醇沉积物的效果。
从泡罩包装中取出五种SH镜片材料：senofilcon A、comfilcon A、balafilcon A、lotrafilcon A和lotrafilcon B（每种镜片类型n = 4），在含有C放射性标记胆固醇的人工泪液中于37°C孵育7天。此后，将镜片储存在保存的生理盐水对照液（Sensitive Eyes Saline Plus）中，或根据制造商的建议，使用揉搓冲洗技术用五种含有不同防腐剂的MPS之一进行清洁（POLYQUAD/ALDOX、聚季铵盐-1/双氯苯双胍己烷、聚季铵盐-1/聚六亚甲基双胍，以及两种仅基于聚六亚甲基双胍的护理液），并在MPS中储存至少6小时。然后用2:1的氯仿：甲醇萃取镜片，在β计数器中进行分析，并测定每片镜片中胆固醇的微克数。
Balafilcon A和senofilcon A镜片材料显示出最高的胆固醇积累量（分别为0.93±0.02微克/片；0.95±0.01微克/片），而lotrafilcon A和lotrafilcon B的沉积量最低（分别为0.37±0.03微克/片；0.47±0.12微克/片）。对于所有镜片材料，用POLYQUAD/ALDOX保存的MPS去除的沉积胆固醇比任何其他测试溶液都多；然而，从单个隐形眼镜中去除的胆固醇污染量仅对balafilcon A和senofilcon A具有统计学意义（分别为P = 0.006和P = 0.042）。敏感眼护理液和其他评估的MPS对胆固醇去除没有显著影响（P>0.05）。
胆固醇去除效果因镜片材料和护理液的组合而异。在5种测试的镜片材料中，只有1种MPS仅对其中2种材料显示出胆固醇沉积的统计学显著降低。
