Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute (TIRI), Tokyo, Japan.
Optical Radiation and Acoustics Technology Group, Tokyo Metropolitan Industrial Technology Research Institute (TIRI), Tokyo, Japan.
J Biomed Mater Res B Appl Biomater. 2019 May;107(4):1011-1023. doi: 10.1002/jbm.b.34194. Epub 2018 Sep 10.
We developed a continuous formation method of cord-like collagen gels comprising fibrils preferentially aligned along the geometrical axes (CCGs). Collagen (2.5%) dissolved in a sodium phosphate buffer containing 280 mM of sodium chloride was introduced into a stainless cylinder (length 52 mm, diameter 2.0 mm) warmed at 38°C at a linear velocity of 2.5 mm/s. This process caused collagen fibril alignments under acute fibril formation in the cylinder, resulting in continuous formation of CCGs. Fibril formation rate, shear rate, and shear duration were substantial factors for successful CCG formation. Outstanding advantages of this method over conventional wet spinning include the capacity of this technique to form aligned fibrils in the entire gels and to control the diameter of cord-like gels over 1 mm. The air-drying of CCGs which were crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxy-succinimide produced dry collagen fibers with cross-sectional areas of 0.0123-0.135 mm . Upon the rewetting of the fibers, they failed at a stress of 54.5 ± 7.8 MPa, which is higher than the mean failure stress of anterior cruciate ligament tissue (13.3-37.8 MPa). These findings indicate that the CCG formation method enables the fabrication of collagen fibers which are potential components of collagen-based artificial tendons. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1011-1023, 2019.
我们开发了一种连续形成线状胶原凝胶的方法，该凝胶由优先沿几何轴排列的纤维组成（CCG）。将溶解在含有 280mM 氯化钠的磷酸钠缓冲液中的 2.5%胶原以 2.5mm/s 的线性速度引入在 38°C 下加热的不锈钢圆柱（长 52mm，直径 2.0mm）中。这个过程导致在圆柱中形成胶原纤维时纤维的排列，从而连续形成 CCG。纤维形成速率、剪切速率和剪切持续时间是成功形成 CCG 的重要因素。与传统湿法纺丝相比，这种方法的突出优点包括该技术能够在整个凝胶中形成排列纤维并控制线状凝胶的直径超过 1mm。用 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺和 N-羟基琥珀酰亚胺交联的 CCG 进行空气干燥，产生具有 0.0123-0.135mm ² 横截面面积的干燥胶原纤维。当纤维重新润湿时，它们在 54.5±7.8MPa 的应力下失效，这高于前交叉韧带组织的平均失效应力（13.3-37.8MPa）。这些发现表明 CCG 形成方法能够制造出潜在的胶原基人工肌腱组成部分的胶原纤维。©2018 年 Wiley 期刊，Inc.J Biomed Mater Res Part B: Appl Biomater 107B:1011-1023，2019 年。
