Department of Biomedical Engineering, Oregon Health & Science University.
Department of Biomedical Engineering, Oregon Health & Science University; Department of Orthopaedics and Rehabilitation, Oregon Health & Science University;
J Vis Exp. 2024 Sep 20(211). doi: 10.3791/67165.
Regenerative biomaterials are designed to facilitate cell-material interactions to guide the repair of damaged tissues and organs. These materials are designed to emulate the biophysical properties of native tissue, providing cellular phenotypic and morphological guidance that contributes to the restoration of the regenerative tissue niche. Collagen, a prevalent extracellular matrix protein, is a common component of these regenerative biomaterials due to its biocompatibility and other favorable properties. The current study describes a novel and straightforward method for the fabrication of engineered nanofibrillar collagen with directed fibril patterning. Through the manipulation of shear stress, temperature, and pH, collagen fibrillogenesis and alignment are precisely controlled without requiring specialized equipment. This approach allows for the creation of nanofibrillar collagen biomaterials that mimic the native structure of tissues exhibiting either anisotropic or isotropic characteristics. The flexibility in collagen nanofibril patterning not only facilitates the study of nanoscale patterning on cell behavior but also offers diverse possibilities for patterned tissue engineering applications.
再生生物材料旨在促进细胞与材料的相互作用，以指导受损组织和器官的修复。这些材料旨在模拟天然组织的生物物理特性，为细胞表型和形态提供指导，有助于再生组织龛的恢复。胶原蛋白是一种常见的细胞外基质蛋白，由于其生物相容性和其他优良特性，是这些再生生物材料的常见成分。本研究描述了一种新颖而简单的方法，用于制造具有定向纤维图案的工程化纳米纤维胶原。通过操纵剪切应力、温度和 pH 值，可以精确控制胶原原纤维的形成和排列，而无需特殊设备。这种方法可以制造出模拟具有各向异性或各向同性特征的组织的天然结构的纳米纤维胶原生物材料。胶原纳米纤维图案化的灵活性不仅有助于研究细胞行为的纳米级图案化，还为图案组织工程应用提供了多种可能性。
