Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China.
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China; Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Ecotoxicol Environ Saf. 2018 Dec 15;165:250-260. doi: 10.1016/j.ecoenv.2018.09.003. Epub 2018 Sep 7.
The remediation and subsequent use of saline-alkaline land are of great significance to ecological environment construction and sustainable agricultural development. Iris halophila Pall. is a salt-tolerant medicinal and ornamental plant, which has good application prospects in the ecological construction of saline-alkaline land; therefore, study of the molecular mechanisms of salt tolerance in I. halophila has important theoretical and practical value. To evaluate the molecular mechanism of the response of I. halophila to salt toxicity, I. halophila seedlings were treated with salt (300 mM NaCl) and subjected to deep RNA sequencing. The clean reads were obtained and assembled into 297,188 unigenes. Among them, 1120 and 100 salt-responsive genes were identified in I. halophila shoots and roots, respectively. Among them, the key flavonoid and lignin biosynthetic genes, hormone signaling genes, sodium/potassium ion transporter genes, and transcription factors were analyzed and summarized. Quantitative reverse-transcription PCR analysis strengthened the reliability of the RNA sequencing results. This work provides an overview of the transcriptomic responses to salt toxicity in I. halophila and identifies the responsive genes that may contribute to its reduced salt toxicity. These results lay an important foundation for further study of the molecular mechanisms of salt tolerance in I. halophila and related species.
盐碱性土地的修复和后续利用对生态环境建设和可持续农业发展具有重要意义。 獐牙菜（Iris halophila Pall.）是一种耐盐药用和观赏植物，在盐碱性土地的生态建设中有很好的应用前景；因此，研究獐牙菜的耐盐分子机制具有重要的理论和实践价值。为了评估獐牙菜对盐毒性响应的分子机制，用盐（300mM NaCl）处理獐牙菜幼苗，并进行深度 RNA 测序。获得清洁读取并组装成 297188 个 unigenes。其中，在獐牙菜的地上部和根部分别鉴定到 1120 个和 100 个盐响应基因。其中，分析和总结了关键类黄酮和木质素生物合成基因、激素信号基因、钠/钾离子转运基因和转录因子。定量逆转录 PCR 分析增强了 RNA 测序结果的可靠性。这项工作为獐牙菜对盐毒性的转录组响应提供了概述，并鉴定了可能有助于其降低盐毒性的响应基因。这些结果为进一步研究獐牙菜及其相关物种的耐盐分子机制奠定了重要基础。
