Li Danqing, Shao Lingmei, Xu Tong, Wang Xiaobin, Zhang Runlong, Zhang Kaijing, Xia Yiping, Zhang Jiaping
Genomics and Genetic Engineering Laboratory of Ornamental Plants, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
Front Genet. 2022 Mar 16;13:841957. doi: 10.3389/fgene.2022.841957. eCollection 2022.
Japanese iris () is a popular perennial ornamental that originated in China; it has a long display period and remains green outdoors throughout the year. winter dormancy characteristics contribute greatly to the evergreenness of herbaceous perennials. Thus, it is crucial to explore the mechanism of winter dormancy in this evergreen herbaceous perennial. Here, we used the hybrid RNA-seq strategy including single-molecule real-time (SMRT) and next-generation sequencing (NGS) technologies to generate large-scale Full-length transcripts to examine the shoot apical meristems of Japanese iris. A total of 10.57 Gb clean data for SMRT and over 142 Gb clean data for NGS were generated. Using hybrid error correction, 58,654 full-length transcripts were acquired and comprehensively analysed, and their expression levels were validated by real-time qPCR. This is the first full-length RNA-seq study in the genus; our results provide a valuable resource and improve understanding of RNA processing in this genus, for which little genomic information is available as yet. In addition, our data will facilitate in-depth analyses of winter dormancy mechanisms in herbaceous perennials, especially evergreen monocotyledons.
鸢尾属植物是一种广受欢迎的多年生观赏植物，原产于中国；它的观赏期长，全年在户外保持绿色。冬季休眠特性对多年生草本植物的常绿性有很大贡献。因此，探索这种常绿多年生草本植物的冬季休眠机制至关重要。在这里，我们使用了包括单分子实时（SMRT）和下一代测序（NGS）技术在内的混合RNA-seq策略，以生成大规模全长转录本，来研究鸢尾属植物的茎尖分生组织。共生成了10.57Gb的SMRT清洁数据和超过142Gb的NGS清洁数据。通过混合纠错，获得了58,654个全长转录本并进行了全面分析，其表达水平通过实时qPCR进行了验证。这是鸢尾属植物的首次全长RNA-seq研究；我们的结果提供了宝贵的资源，并增进了对该属植物RNA加工的理解，目前该属植物的基因组信息还很少。此外，我们的数据将有助于深入分析多年生草本植物，特别是常绿单子叶植物的冬季休眠机制。
