Jiangsu Key Laboratory for Bioresources of Saline Solis, Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.
Jiangsu Key Laboratory for Bioresources of Saline Solis, Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China.
Plant Physiol Biochem. 2018 Apr;125:1-12. doi: 10.1016/j.plaphy.2018.01.019. Epub 2018 Jan 31.
As a halophyte, Iris lactea var. chinensis (I. lactea var. chinensis) is widely distributed and has good drought and heavy metal resistance. Moreover, it is an excellent ornamental plant. I. lactea var. chinensis has extensive application prospects owing to the global impacts of salinization. To better understand its molecular mechanism involved in salt resistance, the de novo sequencing, assembly, and analysis of I. lactea var. chinensis roots' transcriptome in response to salt-stress conditions was performed. On average, 74.17% of the clean reads were mapped to unigenes. A total of 121,093 unigenes were constructed and 56,398 (46.57%) were annotated. Among these, 13,522 differentially expressed genes (DEGs) were identified between salt-treated and control samples Compared to the transcriptional level of control, 7037 DEGs were up-regulated and 6539 down-regulated. In addition, 129 up-regulated and 1609 down-regulated genes were simultaneously detected in all three pairwise comparisons between control and salt-stressed libraries. At least 247 and 250 DEGs encoding transcription factors and transporter proteins were identified. Meanwhile, 130 DEGs regarding reactive oxygen species (ROS) scavenging system were also summarized. Based on real-time quantitative RT-PCR, we verified the changes in the expression patterns of 10 unigenes. Our study identified potential salt-responsive candidate genes and increased the understanding of halophyte responses to salinity stress.
作为一种盐生植物，中华灯心草（I. lactea var. chinensis）分布广泛，具有良好的耐旱性和重金属抗性。此外，它还是一种优良的观赏植物。由于全球盐渍化的影响，中华灯心草具有广泛的应用前景。为了更好地了解其耐盐分子机制，对盐胁迫条件下中华灯心草根转录组的从头测序、组装和分析进行了研究。平均而言，74.17%的清洁读取序列可以比对到 unigenes。共构建了 121093 个 unigenes，其中 56398 个（46.57%）被注释。在盐处理和对照样品之间共鉴定到 13522 个差异表达基因（DEGs）。与对照的转录水平相比，有 7037 个 DEGs 上调，6539 个下调。此外，在对照和盐胁迫文库之间的所有三个两两比较中，同时检测到 129 个上调和 1609 个下调基因。鉴定到至少 247 个和 250 个编码转录因子和转运蛋白的 DEGs。同时，还总结了 130 个与活性氧（ROS）清除系统相关的 DEGs。通过实时定量 RT-PCR，我们验证了 10 个 unigenes表达模式的变化。本研究鉴定了潜在的盐响应候选基因，增加了对盐生植物响应盐胁迫的理解。
