Shi Jia, Gao Shu-Tao, Lv Zheng-Tao, Sheng Wei-Bin, Kang Hao
Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Department of Spine Surgery, The First Affiliate Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Medicine (Baltimore). 2018 Sep;97(36):e12235. doi: 10.1097/MD.0000000000012235.
The single nucleotide polymorphism (SNP) rs12885713 of calmodulin 1 gene (CALM1) has been reported to involve in the etiology of osteoarthritis (OA) in several association studies with limited sample size and conflicting results. The purpose of the present systematic review and meta-analysis was to evaluate and synthesize the currently available data on the correlation between rs12885713 and OA susceptibility.
Six electronic databases including PubMed, EMBASE, ISI Web of Science, CNETRAL, CNKI, and Wanfang were systematically retrieved to identify relevant observational articles published before October 2017. Summary odds ratios (ORs) and corresponding 95% confidence intervals (95% CIs) were calculated to indicate the association between CALM1 polymorphism and OA. Risk of bias was assessed through the Newcastle-Ottawa Scale. Predetermined subgroups and sensitivity analyses were performed using the RevMan 5.3 software. Publication bias was evaluated by Egger and Begg tests.
Overall, 5 case-control studies involving 2183 OA patients 2654 healthy control subjects satisfied the meta-analysis. Recessive model was confirmed to be the best-matching genetic model (TT + TC versus CC). The pooled outcomes indicated that rs12885713 SNP was not significantly associated with OA vulnerability (OR 1.11, 95% CI 0.97, 1.27; P = .12). When stratified by different genders, OA sites, and population descents respectively, still non-significant associations were found.
Based on the findings of our present study, the rs12885713 polymorphism of CALM1 did not appear to be associated with OA predisposition.
钙调蛋白1基因（CALM1）的单核苷酸多态性（SNP）rs12885713在一些样本量有限且结果相互矛盾的关联研究中被报道与骨关节炎（OA）的病因有关。本系统评价和荟萃分析的目的是评估和综合目前关于rs12885713与OA易感性之间相关性的现有数据。
系统检索包括PubMed、EMBASE、ISI Web of Science、CNETRAL、CNKI和万方在内的6个电子数据库，以识别2017年10月之前发表的相关观察性文章。计算汇总比值比（OR）和相应的95%置信区间（95%CI），以表明CALM1基因多态性与OA之间的关联。通过纽卡斯尔-渥太华量表评估偏倚风险。使用RevMan 5.3软件进行预定的亚组分析和敏感性分析。通过Egger检验和Begg检验评估发表偏倚。
总体而言，5项病例对照研究涉及2183例OA患者和2654例健康对照者，满足荟萃分析要求。隐性模型被确认为最佳匹配遗传模型（TT + TC对CC）。汇总结果表明，rs12885713 SNP与OA易感性无显著关联（OR 1.11，95%CI 0.97，1.27；P = 0.12）。分别按不同性别、OA部位和人群血统进行分层时，仍未发现显著关联。
基于我们目前的研究结果，CALM1的rs12885713多态性似乎与OA易感性无关。
