Parrilla-Almansa Andrés, García-Carrillo Nuria, Ros-Tárraga Patricia, Martínez Carlos M, Martínez-Martínez Francisco, Meseguer-Olmo Luis, De Aza Piedad N
Image Diagnostic Service, Virgen de la Arrixaca University Hospital, UCAM-Universidad Catolica San Antonio de Murcia, Guadalupe, 30107 Murcia, Spain.
Preclinical Imaging Unit, Laboratory Animal Service, University of Murcia, 30107 Murcia, Spain.
Materials (Basel). 2018 Sep 1;11(9):1580. doi: 10.3390/ma11091580.
The aim of this study was to manufacture and evaluate the effect of a biphasic calcium silicophosphate (CSP) scaffold ceramic, coated with a natural demineralized bone matrix (DBM), to evaluate the efficiency of this novel ceramic material in bone regeneration. The DBM-coated CSP ceramic was made by coating a CSP scaffold with gel DBM, produced by the partial sintering of different-sized porous granules. These scaffolds were used to reconstruct defects in rabbit tibiae, where CSP scaffolds acted as the control material. Micro-CT and histological analyses were performed to evaluate new bone formation at 1, 3, and 5 months post-surgery. The present research results showed a correlation among the data obtained by micro-CT and the histomorphological results, the gradual disintegration of the biomaterial, and the presence of free scaffold fragments dispersed inside the medullary cavity occupied by hematopoietic bone marrow over the 5-month study period. No difference was found between the DBM-coated and uncoated implants. The new bone tissue inside the implants increased with implantation time. Slightly less new bone formation was observed in the DBM-coated samples, but it was not statistically significant. Both the DBM-coated and the CSP scaffolds gave excellent bone tissue responses and good osteoconductivity.
本研究的目的是制备并评估一种涂覆有天然脱矿骨基质（DBM）的双相磷酸硅钙（CSP）支架陶瓷的效果，以评估这种新型陶瓷材料在骨再生中的效率。DBM涂覆的CSP陶瓷是通过用凝胶DBM涂覆CSP支架制成的，凝胶DBM由不同尺寸的多孔颗粒部分烧结产生。这些支架用于修复兔胫骨缺损，其中CSP支架作为对照材料。在术后1、3和5个月进行显微CT和组织学分析，以评估新骨形成情况。本研究结果表明，在5个月的研究期内，显微CT获得的数据与组织形态学结果、生物材料的逐渐分解以及分散在由造血骨髓占据的髓腔内的游离支架碎片之间存在相关性。DBM涂覆和未涂覆的植入物之间未发现差异。植入物内的新骨组织随植入时间增加。在DBM涂覆的样本中观察到的新骨形成略少，但无统计学意义。DBM涂覆的支架和CSP支架均产生了良好的骨组织反应和良好的骨传导性。
