Gazor Rouhallah, Asgari Mehrdad, Abdollajhifar Mohammad-Amin, Kiani Pejman, Zare Fatemeh, Fadaei Fathabady Fatemeh, Norouzian Mohsen, Amini Abdollah, Khosravipour Armin, Atashgah Rahimeh B, Kazemi Mahsa, Chien Sufan, Bayat Mohammad
Department of Anatomy and Cell Biology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; And Department of Maxillofacial Radiology, Guilan University of Medical Sciences, Rasht, Guilan, Iran.
J Lasers Med Sci. 2021 Aug 2;12:e41. doi: 10.34172/jlms.2021.41. eCollection 2021.
The ability of simultaneous treatment of critical-sized femoral defects (CSFDs) with photobiomodulation (PBM) and demineralized bone matrix (DBM) with or without seeded adipose-derived stem cells (ASCs) to induce bone reconstruction in ovariectomized induced osteoporotic (OVX) rats was investigated. The OVX rats with CSFD were arbitrarily separated into 6 groups: control, scaffold (S, DBM), S + PBM, S + alendronate (ALN), S + ASCs, and S + PBM + ASCs. Each group was assessed by cone beam computed tomography (CBCT) and histological examinations. In the fourth week, CBCT and histological analyses revealed that the largest volume of new bone formed in the S + PBM and S + PBM + ASC groups. The S + PBM treatment relative to the S and S + ALN treatments remarkably reduced the CSFD (Mann-Whitney test, = 0.009 and = 0.01). Furthermore, S + PBM + ASCs treatment compared to the S and S + ALN treatments significantly decreased CSFD (Mann Whitney test, = 0.01). In the eighth week, CBCT analysis showed that extremely enhanced bone regeneration occurred in the CSFD of the S + PBM group. Moreover, the CSFD in the S + PBM group was substantially smaller than S, S + ALN and S + ASCs groups (Mann Whitney test, = 0.01, = 0.02 and = 0.009). Histological observations showed more new bone formation in the treated CSFD of S + PBM + ASCs and S + PBM groups. The PBM plus DBM with or without ASCs significantly enhanced bone healing in the CSFD in OVX rats compared to control, DBM alone, and ALN plus DBM groups. The PBM plus DBM with or without ASCs significantly decreased the CSFD area compared to either the solo DBM or ALN plus DBM treatments.
研究了光生物调节（PBM）联合脱矿骨基质（DBM），以及联合或不联合接种脂肪来源干细胞（ASC）同时治疗去卵巢诱导骨质疏松（OVX）大鼠临界尺寸股骨缺损（CSFD）以诱导骨重建的能力。将患有CSFD的OVX大鼠随机分为6组：对照组、支架组（S，DBM）、S + PBM组、S +阿仑膦酸钠（ALN）组、S + ASC组和S + PBM + ASC组。每组通过锥束计算机断层扫描（CBCT）和组织学检查进行评估。在第四周，CBCT和组织学分析显示，S + PBM组和S + PBM + ASC组形成的新骨体积最大。与S组和S + ALN组相比，S + PBM治疗显著减小了CSFD（曼-惠特尼检验，P = 0.009和P = 0.01）。此外，与S组和S + ALN组相比，S + PBM + ASC治疗显著减小了CSFD（曼-惠特尼检验，P = 0.01）。在第八周，CBCT分析显示，S + PBM组的CSFD中出现了极其增强的骨再生。此外，S + PBM组的CSFD明显小于S组、S + ALN组和S + ASC组（曼-惠特尼检验，P = 0.01、P = 0.02和P = 0.009）。组织学观察显示，S + PBM + ASC组和S + PBM组治疗后的CSFD中有更多新骨形成。与对照组、单独DBM组和ALN + DBM组相比，PBM联合DBM，无论是否联合ASC，均显著增强了OVX大鼠CSFD中的骨愈合。与单独DBM或ALN + DBM治疗相比，PBM联合DBM，无论是否联合ASC，均显著减小了CSFD面积。
