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来自欧洲国家的草豌豆种子的抗氧化潜力

Antioxidant Potential of Grass Pea Seeds from European Countries.

作者信息

Rybiński Wojciech, Karamać Magdalena, Sulewska Katarzyna, Börner Andreas, Amarowicz Ryszard

机构信息

Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.

Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland.

出版信息

Foods. 2018 Sep 1;7(9):142. doi: 10.3390/foods7090142.

Abstract

Phenolic compounds were extracted from seeds of 30 varieties of grass pea () into 80% (/) methanol. The total phenolics compounds content of the extracts and their antioxidant activity were determined using Folin-Ciocalteu's phenol reagent and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric-reducing antioxidant power (FRAP) methods, respectively. Total phenolic contents ranged from 1.88 to 7.12 mg/g extract and 20.3 to 70.3 mg/100 g seeds. The extracts and seeds were characterized using Trolox equivalent antioxidant capacity values of 0.015⁻0.037 mmol Trolox/g extract and 0.158⁻0.372 mmol Trolox/100 g seeds, and FRAP values of 0.045⁻0.120 mmol Fe/g extract and 0.487⁻1.189 Fe/100 g seeds. The total phenolics content of grass pea extract was correlated with the results of the ABTS ( = 0.881) and FRAP ( = 0.781) assays. The same correlation was observed between the results of both assays ( = 0.842). Two derivatives of -coumaric acid were the dominant phenolic compounds of the Derek cultivar of grass pea.

摘要

从30个草豌豆品种的种子中提取酚类化合物至80%(体积分数)甲醇中。分别使用福林-西奥尔特试剂、2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)和铁还原抗氧化能力(FRAP)方法测定提取物中总酚类化合物含量及其抗氧化活性。总酚含量范围为1.88至7.12毫克/克提取物以及20.3至70.3毫克/100克种子。提取物和种子的特征在于其特罗克斯等效抗氧化能力值分别为0.015⁻0.037毫摩尔特罗克斯/克提取物和0.158⁻0.372毫摩尔特罗克斯/100克种子,以及FRAP值分别为0.045⁻0.120毫摩尔铁/克提取物和0.487⁻1.189铁/100克种子。草豌豆提取物的总酚含量与ABTS(r = 0.881)和FRAP(r = 0.781)测定结果相关。两种测定结果之间也观察到相同的相关性(r = 0.842)。对香豆酸的两种衍生物是草豌豆德里克品种的主要酚类化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b641/6164006/8bfcc4967660/foods-07-00142-g001.jpg

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