Rybiński Wojciech, Karamać Magdalena, Sulewska Katarzyna, Börner Andreas, Amarowicz Ryszard
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland.
Foods. 2018 Sep 1;7(9):142. doi: 10.3390/foods7090142.
Phenolic compounds were extracted from seeds of 30 varieties of grass pea () into 80% (/) methanol. The total phenolics compounds content of the extracts and their antioxidant activity were determined using Folin-Ciocalteu's phenol reagent and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric-reducing antioxidant power (FRAP) methods, respectively. Total phenolic contents ranged from 1.88 to 7.12 mg/g extract and 20.3 to 70.3 mg/100 g seeds. The extracts and seeds were characterized using Trolox equivalent antioxidant capacity values of 0.015⁻0.037 mmol Trolox/g extract and 0.158⁻0.372 mmol Trolox/100 g seeds, and FRAP values of 0.045⁻0.120 mmol Fe/g extract and 0.487⁻1.189 Fe/100 g seeds. The total phenolics content of grass pea extract was correlated with the results of the ABTS ( = 0.881) and FRAP ( = 0.781) assays. The same correlation was observed between the results of both assays ( = 0.842). Two derivatives of -coumaric acid were the dominant phenolic compounds of the Derek cultivar of grass pea.
从30个草豌豆品种的种子中提取酚类化合物至80%(体积分数)甲醇中。分别使用福林-西奥尔特试剂、2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)和铁还原抗氧化能力(FRAP)方法测定提取物中总酚类化合物含量及其抗氧化活性。总酚含量范围为1.88至7.12毫克/克提取物以及20.3至70.3毫克/100克种子。提取物和种子的特征在于其特罗克斯等效抗氧化能力值分别为0.015⁻0.037毫摩尔特罗克斯/克提取物和0.158⁻0.372毫摩尔特罗克斯/100克种子,以及FRAP值分别为0.045⁻0.120毫摩尔铁/克提取物和0.487⁻1.189铁/100克种子。草豌豆提取物的总酚含量与ABTS(r = 0.881)和FRAP(r = 0.781)测定结果相关。两种测定结果之间也观察到相同的相关性(r = 0.842)。对香豆酸的两种衍生物是草豌豆德里克品种的主要酚类化合物。