Department of Clinical Chemistry and Laboratory Medicine, Saarland University Hospital, Building 57, 66424 Homburg/Saar, Germany.
Nutrients. 2018 Sep 1;10(9):1209. doi: 10.3390/nu10091209.
Plasma choline shows associations with plasma glucose and lipids. We studied changes of choline metabolites after oral glucose tolerance test (OGTT) and fat tolerance test (OFTT). Eighteen healthy subjects (mean age 54.3 years; BMI 26.8 kg/m²) underwent 2 tests. First, OFTT (80 g fat) was applied and blood was collected at baseline and 4 h after OFTT. Seven days later, 75 g glucose was applied and blood was collected at baseline and 2 h after OGTT. Plasma concentrations of choline, betaine, trimethylamine N-oxide (TMAO), dimethylglycine, S-adenosylmethionine (SAM), lipids and glucose were measured. After OFTT, plasma choline declined (10.6 to 9.2 µmol/L; = 0.004), betaine declined (33.4 to 31.7 µmol/L; = 0.003), TMAO slightly increased (4.1 to 5.6 µmol/L; = 0.105), glucose declined (5.39 to 4.98 mmol/L; < 0.001), and triglycerides increased (1.27 to 2.53 mmol/L; < 0.001). After OGTT, plasma choline increased (10.1 to 11.1 µmol/L; < 0.001), TMAO declined (4.0 to 3.5 µmol/L; = 0.029), dimethylglycine declined (2.0 to 1.7 µmol/L; = 0.005), SAM declined (103 to 96 nmol/L; = 0.041), but betaine, glucose, and SAM were unchanged. In conclusion, OFTT lowered plasma betaine and choline and caused heterogeneous changes in plasma TMAO. OGTT reduced the flow of methyl groups and plasma TMAO.
血浆胆碱与血糖和血脂有关。我们研究了口服葡萄糖耐量试验（OGTT）和脂肪耐量试验（OFTT）后胆碱代谢物的变化。18 名健康受试者（平均年龄 54.3 岁；BMI 26.8 kg/m²）接受了 2 项测试。首先，进行了 OFTT（80 g 脂肪），并在 OFTT 后 4 小时采集血液。7 天后，给予 75 g 葡萄糖，并在 OGTT 后 2 小时采集血液。测量了血浆胆碱、甜菜碱、三甲胺 N-氧化物（TMAO）、二甲基甘氨酸、S-腺苷甲硫氨酸（SAM）、脂质和葡萄糖的浓度。OFTT 后，血浆胆碱下降（10.6 至 9.2 µmol/L；= 0.004），甜菜碱下降（33.4 至 31.7 µmol/L；= 0.003），TMAO 略有增加（4.1 至 5.6 µmol/L；= 0.105），血糖下降（5.39 至 4.98 mmol/L；< 0.001），甘油三酯增加（1.27 至 2.53 mmol/L；< 0.001）。OGTT 后，血浆胆碱增加（10.1 至 11.1 µmol/L；< 0.001），TMAO 下降（4.0 至 3.5 µmol/L；= 0.029），二甲基甘氨酸下降（2.0 至 1.7 µmol/L；= 0.005），SAM 下降（103 至 96 nmol/L；= 0.041），但甜菜碱、葡萄糖和 SAM 没有变化。总之，OFTT 降低了血浆甜菜碱和胆碱，并导致血浆 TMAO 发生不均匀变化。OGTT 减少了甲基供体的流动和血浆 TMAO。
