Wang Jing-Jing, Hao Wen-Fang, Zhang Ji-Wen, Shu Zhi-Ming
College of Life Sciences， Northwest Agriculture and Forestry University of Science and Technology， Yangling 712100， China.
College of Chemistry & Pharmacy, Northwest Agriculture and Forestry University of Science and Technology， Yangling 712100， China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(15):3163-3170. doi: 10.19540/j.cnki.cjcmm.20180521.002.
Combined headspace solid-phase microextraction with gas chromatography-mass spectrometry (HS-SPME-GC-MS), this paper studied the volatiles of Artemisiae Argyi Folium that respectively collected from Kongdong, Huanglong, Ankang, Qichun and Tongbai，and then explored the influence of climatic factors on the main components of the volatiles. The results showed that 54, 52, 57, 47 and 55 compounds were respectively identified from the leaves from five regions, mainly consist of monoterpenes, sesquiterpenoids and their oxygen-containing derivatives, a few aldehydes, alkanes and benzenes. It is different that the volatiles of Artemisiae Argyi Folium that collected in different habitats, neither composition nor content. There are total 18 kinds of compounds that exist in all five leaves. Eucalyptol, camphor, terpinen-4-ol, eugenol and caryophyllene could be considered to be the main conponents because of their efficacy or toxicity and relatively high content. Grey correlation analysis shows that the annual lowest temperature and annual sunshine duration have a great influence on the content of eucalyptol and camphor; the effect of annual precipitation and annual accumulated temperature on the content of terpinen-4-ol and caryophyllene was relatively significant; while the annual sunshine duration and the annual highest temperature are the main influencing factors for eugenol. The research suggests that long-day and extreme temperature may be more conductive to the accumulation of volatiles. According to the laws of effect of climatic factors on volatiles, we can optimize the cultivation conditions and select suitable locality to improve the quality of Artemisiae Argyi Folium.
本文采用顶空固相微萃取-气相色谱-质谱联用（HS-SPME-GC-MS）技术，研究了分别采自崆峒、黄龙、安康、蕲春和桐柏的艾叶挥发物，并探讨了气候因子对挥发物主要成分的影响。结果表明，从五个地区的叶片中分别鉴定出54、52、57、47和55种化合物，主要由单萜类、倍半萜类及其含氧衍生物组成，还有少量醛类、烷烃类和苯类。不同产地艾叶的挥发物不同，无论是组成还是含量。五个叶片中均存在的化合物共有18种。桉叶油醇、樟脑、萜品-4-醇、丁香酚和石竹烯因其功效或毒性以及相对较高的含量，可被视为主要成分。灰色关联分析表明，年最低气温和年日照时长对桉叶油醇和樟脑含量影响较大；年降水量和年积温对萜品-4-醇和石竹烯含量的影响较为显著；而年日照时长和年最高气温是丁香酚的主要影响因素。研究表明，长日照和极端温度可能更有利于挥发物的积累。根据气候因子对挥发物的影响规律，可以优化栽培条件并选择合适的地点来提高艾叶的品质。
