Gongbao Dong-Zhi, Luo Qing-Fang, Yu Yang-Yang, Wencheng Dang-Zhi, Zeng Shang-Yu, Ganghuan Chen-Lei, Zhang Yi
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine， Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2018 Aug;43(16):3368-3375. doi: 10.19540/j.cnki.cjcmm.20180419.009.
To explore the medication regularity of Tibetan medicine in the treatment of spleen and stomach diseases, analyze the potential drug targets and interactions of the prescriptions, and reveal the mechanism of Tibetan medicine in the treatment of spleen and stomach diseases. The prescriptions in Tibetan medicine for treatment of spleen and stomach diseases were collected, and Traditional Chinese Medicine Inheritance Support System (TCMISS) was used to analyze the association rules between the herbs and discover the core herbs and new prescriptions. The integrated pharmacology platform V1.0 software was used to construct "herb-compound-target" network and investigate the interactions between various herbs and related pathways of Tibetan medicine Wuwei Shiliu powder in the treatment of spleen and stomach diseases. Among the 216 prescriptions of Tibetan medicine in the treatment of spleen and stomach diseases, pomegranate seed was used at a highest frequency (118 times), followed by white cardamom (107 times) and comatose (107 times). 12 new prescriptions were evolved by using the association rules (support>=34%, confidence>=0.85). 5 242 related drug targets and 20 related pathways were obtained from classic formula Wuwei Shiliu Powder (FDR<0.01). It was proposed that Tibetan medicine treatment for spleen and stomach diseases was mainly based on proliferation of "stomach fire" and the main drugs were for regulating Qi-flowing for strengthening spleen. The mechanism may be associated with regulation of digestive juice secretion, proton pump, mitochondria, regulation of intestinal digestion and immunity, the body's immunity to microorganisms function and other multiple targets and pathways to achieve the joint intervention.
为探讨藏药治疗脾胃疾病的用药规律，分析方剂潜在的药物靶点及相互作用，揭示藏药治疗脾胃疾病的作用机制。收集藏药治疗脾胃疾病的方剂，运用中医传承辅助系统（TCMISS）分析药物之间的关联规则，挖掘核心药物及新方剂。采用整合药理学平台V1.0软件构建“药-方-靶”网络，探究藏药五味十六散治疗脾胃疾病的各味药物之间的相互作用及相关通路。在藏药治疗脾胃疾病的216首方剂中，石榴籽使用频次最高（118次），其次是白豆蔻（107次）和昏迷（107次）。运用关联规则（支持度≥34%，置信度≥0.85）演化得到12首新方剂。从经典方剂五味十六散中获得5242个相关药物靶点及20条相关通路（FDR<0.01）。提出藏药治疗脾胃疾病主要以“胃火”亢盛立论，用药以理气健脾为主。其作用机制可能与调节消化液分泌、质子泵、线粒体、调节肠道消化与免疫、机体对微生物免疫功能等多个靶点及通路共同干预有关。
