Mizuguchi J, Tsang W, Morrison S L, Beaven M A, Paul W E
J Immunol. 1986 Oct 1;137(7):2162-7.
Increases in intracellular free calcium concentration ((Ca2+)i) were observed in response to anti-immunoglobulin (Ig) antibodies in each of six B cell tumors or B cell hybridomas bearing mu or delta chains on their cell surface. The BAL17 cell line, bearing mu and delta chains on its surface, behaved similarly to mature B cells in the following respects. Anti-IgM and anti-IgD antibodies caused increases in (Ca2+)i and inositol phospholipid metabolism; the initial increases in (Ca2+)i were derived partly from an intracellular Ca2+ pool; lipopolysaccharide, phorbol myristate acetate (PMA), B cell stimulatory factor-1, and antibodies to class I and class II major histocompatibility molecules and to the Fc gamma receptor failed to cause increases in (Ca2+)i or in inositol phospholipid metabolism; and increases in (Ca2+)i and inositol phospholipid metabolism in response to anti-Ig were inhibited by pretreatment with PMA. Furthermore A20, an IgG2a bearing lymphoma, showed increases in (Ca2+)i in response to anti-IgG2a, and a lymphoma cell line (6G8-2E10) expressing membrane IgG2b as a result of DNA-mediated transfer of the gamma 2b H chain gene, showed increases in (Ca2+)i in response to anti-IgG2b. These results indicate that Ig-bearing lymphomas display early events in B cell activation after receptor cross-linkage and can be used for detailed studies of the activation process.
在六种细胞表面带有μ链或δ链的B细胞肿瘤或B细胞杂交瘤中，均观察到抗免疫球蛋白（Ig）抗体可使细胞内游离钙浓度（(Ca2+)i）升高。表面带有μ链和δ链的BAL17细胞系在以下方面表现得与成熟B细胞相似。抗IgM和抗IgD抗体可使(Ca2+)i和肌醇磷脂代谢增加；(Ca2+)i的初始升高部分源于细胞内钙库；脂多糖、佛波酯肉豆蔻酸酯（PMA）、B细胞刺激因子-1、针对I类和II类主要组织相容性分子以及Fcγ受体的抗体均未能使(Ca2+)i或肌醇磷脂代谢增加；用PMA预处理可抑制抗Ig引起的(Ca2+)i和肌醇磷脂代谢增加。此外，携带IgG2a的淋巴瘤A20对抗IgG2a有反应，(Ca2+)i升高；通过DNA介导的γ2b重链基因转移而表达膜IgG2b的淋巴瘤细胞系（6G8-2E10）对抗IgG2b有反应，(Ca2+)i升高。这些结果表明，携带Ig的淋巴瘤在受体交联后表现出B细胞活化的早期事件，可用于活化过程的详细研究。
