Genes and Dynamics of Memory Systems, Brain Plasticity Unit, CNRS, ESPCI Paris, PSL Research University, 75005 Paris, France.
Genes and Dynamics of Memory Systems, Brain Plasticity Unit, CNRS, ESPCI Paris, PSL Research University, 75005 Paris, France
J Neurosci. 2018 Oct 24;38(43):9202-9214. doi: 10.1523/JNEUROSCI.0876-18.2018. Epub 2018 Sep 10.
It was proposed that the gene () is required for consolidation of aversive memory in the dorsal paired medial (DPM) neurons, a pair of large neurons that broadly innervate the mushroom bodies (MB), the fly center for olfactory learning and memory (Waddell et al., 2000). Yet, a conditional analysis showed that it was not possible to rescue the memory deficit of null mutant flies when expression was restored only in the adult (DeZazzo et al., 1999), which led the authors to suggest that might be involved in the development of brain structures that normally promote adult olfactory memory. To further investigate temporal and spatial requirements of Amnesiac (AMN) peptide in memory, we used RNA interference in combination with conditional drivers. Experiments were conducted either in both sexes, or in either sexes. Our data show that acute modulation of expression in adult DPM neurons does not impact memory. We further show that expression is required for normal development of DPM neurons. Detailed enhancer trap analyses suggest that transcription unit contains two distinct enhancers, one specific of DPM neurons, and the other specific of α/β MB neurons. This prompted us to investigate extensively the role of AMN in the adult MB. Together, our results demonstrate that is acutely required in adult α/β MB neurons for middle-term and long-term memory. The data thus establish that plays two distinct roles. Its expression is required in DPM neurons for their development, and in adult MB for olfactory memory. The gene encodes a neuropeptide whose expression was proposed to be required for consolidation of aversive memory in the dorsal paired medial (DPM) neurons, a pair of large neurons that broadly innervate the mushroom bodies (MB), the olfactory memory center. Here, we investigated temporal and spatial requirement using conditional tools that allowed us to manipulate its expression in selected neurons. This work leads to a complete reassessment of the role of in brain development and memory. We show that is required for two distinct processes: for normal development of DPM neurons, and in adult MB for memory.
有人提出，基因 () 是巩固 dorsal paired medial (DPM) 神经元中厌恶记忆所必需的，DPM 神经元是一对广泛支配蘑菇体 (MB) 的大型神经元，是苍蝇嗅觉学习和记忆的中心 (Waddell 等人，2000)。然而，条件分析表明，当仅在成年时恢复 的表达时，不可能挽救 缺失突变体苍蝇的记忆缺陷 (DeZazzo 等人，1999)，这导致作者提出 可能参与促进成年嗅觉记忆的大脑结构的发育。为了进一步研究 Amnesiac (AMN) 肽在记忆中的时间和空间要求，我们使用 RNA 干扰结合条件驱动程序。实验在两性中进行，或在两性中的任一种性别中进行。我们的数据表明，急性调节成年 DPM 神经元中的 表达不会影响记忆。我们进一步表明， 表达是 DPM 神经元正常发育所必需的。详细的增强子陷阱分析表明， 转录单元包含两个不同的增强子，一个是 DPM 神经元特异性的，另一个是 α/β MB 神经元特异性的。这促使我们广泛研究 AMN 在成年 MB 中的作用。总之，我们的结果表明， 在成年 α/β MB 神经元中急性需要用于中期和长期记忆。这些数据因此证明 具有两个不同的作用。它的表达在 DPM 神经元中对于它们的发育是必需的，并且在成年 MB 中对于嗅觉记忆是必需的。基因编码一种神经肽，其表达被提议是巩固 dorsal paired medial (DPM) 神经元中厌恶记忆所必需的，DPM 神经元是一对广泛支配蘑菇体 (MB) 的大型神经元，是苍蝇嗅觉学习和记忆的中心。在这里，我们使用允许我们在选定神经元中操纵其表达的条件工具来研究 的时空要求。这项工作导致对 在大脑发育和记忆中的作用的全面重新评估。我们表明， 对于两个不同的过程是必需的：DPM 神经元的正常发育，以及成年 MB 中的记忆。
