用于储存式钙内流的锰淬灭测定法。
Mn Quenching Assay for Store-Operated Calcium Entry.
作者信息
Pan Zui, Choi Sangyong, Luo Yanhong
机构信息
College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA.
Department of Endocrinology, The Children's Hospital of Chongqing Medical University, Chongqing, P. R. China.
出版信息
Methods Mol Biol. 2018;1843:55-62. doi: 10.1007/978-1-4939-8704-7_4.
Store-operated Ca entry (SOCE) pathway plays important roles in many cellular processes, which is largely studied by using fluorescent Ca indicator, Fura-2. Extracellular Mn is able to cross the plasma membrane through SOCE and quenches the fluorescence signals from Fura-2. Thus, the fluorescence quenching rate by Mn composes a convenient assay to monitor the extent of SOCE. This chapter describes an experimental method of Mn quenching assay for both cultured esophageal epithelial and skeletal muscle cells. It also explains how to perform a quantitative analysis of graded SOCE.
储存式钙内流（SOCE）途径在许多细胞过程中发挥着重要作用，目前主要通过使用荧光钙指示剂Fura-2进行研究。细胞外的锰能够通过SOCE穿过质膜，并淬灭Fura-2的荧光信号。因此，锰引起的荧光淬灭速率构成了一种方便的检测方法，用于监测SOCE的程度。本章描述了一种用于培养的食管上皮细胞和骨骼肌细胞的锰淬灭检测实验方法。它还解释了如何对分级SOCE进行定量分析。
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