油酸、棕榈酸和硬脂酸在培养的仓鼠肝细胞中的代谢命运。
Metabolic fate of oleic acid, palmitic acid and stearic acid in cultured hamster hepatocytes.
作者信息
Bruce J S, Salter A M
机构信息
Department of Applied Biochemistry and Food Science, University of Nottingham, Loughborough, Leicestershire, U.K.
出版信息
Biochem J. 1996 Jun 15;316 ( Pt 3)(Pt 3):847-52. doi: 10.1042/bj3160847.
Unlike other saturated fatty acids, dietary stearic acid does not appear to raise plasma cholesterol. The reason for this remains to be established, although it appears that it must be related to inherent differences in the metabolism of the fatty acid. In the present study, we have looked at the metabolism of palmitic acid and stearic acid, in comparison with oleic acid, by cultured hamster hepatocytes. Stearic acid was taken up more slowly and was poorly incorporated into both cellular and secreted triacylglycerol. Despite this, stearic acid stimulated the synthesis and secretion of triacylglycerol to the same extent as the other fatty acids. Incorporation into cellular phospholipid was lower for oleic acid than for palmitic acid and stearic acid. Desaturation of stearic acid, to monounsaturated fatty acid, was found to be greater than that of palmitic acid. Oleic acid produced from stearic acid was incorporated into both triacylglycerol and phospholipid, representing 13% and 6% respectively of the total after a 4 h incubation. Significant proportions of all of the fatty acids were oxidized, primarily to form ketone bodies, but by 8 h more oleic acid had been oxidized compared with palmitic acid and stearic acid.
与其他饱和脂肪酸不同，膳食中的硬脂酸似乎不会升高血浆胆固醇。尽管其原因似乎一定与该脂肪酸代谢的内在差异有关，但仍有待确定。在本研究中，我们通过培养的仓鼠肝细胞，研究了棕榈酸和硬脂酸与油酸相比的代谢情况。硬脂酸的摄取较慢，且很少掺入细胞内和分泌的三酰甘油中。尽管如此，硬脂酸刺激三酰甘油合成和分泌的程度与其他脂肪酸相同。油酸掺入细胞磷脂的量低于棕榈酸和硬脂酸。发现硬脂酸去饱和生成单不饱和脂肪酸的程度大于棕榈酸。由硬脂酸产生的油酸分别掺入三酰甘油和磷脂中，孵育4小时后分别占总量的13%和6%。所有脂肪酸都有相当比例被氧化，主要生成酮体，但到8小时时，与棕榈酸和硬脂酸相比，被氧化的油酸更多。
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