Department of Plant Biotechnology, University of Guilan, University Campus 2, Rasht, Iran.
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Mol Biotechnol. 2018 Dec;60(12):863-878. doi: 10.1007/s12033-018-0120-5.
Development of efficient, easy, and safe gene delivery methods is of great interest in the field of plant biotechnology. Considering the limitations of the usual transfection methods (such as transgene size and plant type), several new techniques have been tested for replacement. The success of some biological and synthetic nanostructures such as cell-penetrating peptides and carbon nanotubes in transferring macromolecules (proteins and nucleic acids) into mammalian cells provoked us to assess the ability of an engineered chimeric peptide and also arginine functionalized single-walled carbon nanotube in gene delivery to intact tobacco (Nicotiana tabacum var. Virginia) root cells. It was suggested that the engineered peptide with its special cationic and hydrophobic domains and the arginine functionalized single-walled carbon nanotube due to its nano-cylindrical shape can pass plant cell barriers while plasmid DNA (which codes green fluorescent protein) has been condensed on them. The success of gene delivery to tobacco root cells was confirmed by fluorescence microscopy and western blotting analysis.
发展高效、简便、安全的基因传递方法是植物生物技术领域的研究热点。鉴于常用转染方法（如转基因大小和植物类型）的局限性，已经测试了几种新技术来替代它们。一些生物和合成纳米结构（如穿透肽和碳纳米管）在将大分子（蛋白质和核酸）递送入哺乳动物细胞方面的成功，促使我们评估一种工程化嵌合肽和精氨酸功能化单壁碳纳米管在将基因递送到完整烟草（Nicotiana tabacum var. Virginia）根细胞中的能力。研究表明，具有特殊阳离子和疏水性结构域的工程化肽和由于其纳米圆柱形形状而具有精氨酸功能的单壁碳纳米管可以穿透植物细胞屏障，而质粒 DNA（编码绿色荧光蛋白）已被它们浓缩。通过荧光显微镜和 Western blot 分析证实了基因传递到烟草根细胞的成功。
