Osmani Zhila, Kulka Marianna
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB T6G 2E1, Canada.
Quantum and Nanotechnologies Research Center, National Research Council Canada, Edmonton, AB T6G 2M9, Canada.
Molecules. 2025 Jan 21;30(3):446. doi: 10.3390/molecules30030446.
Nanoparticle (NP)-mediated gene delivery offers a promising alternative to traditional methods in plant biotechnology, facilitating genetic transformations with enhanced precision and efficiency. This review discusses key factors influencing NP efficacy, including plant cell wall composition, DNA/NP ratios, exposure time, cargo loading, and post-transformation assessments. We explore the challenges of NP cytotoxicity, transformation efficiency, and regeneration while addressing environmental impacts and regulatory considerations. We emphasize the potential for stimulus-responsive NPs and scalable delivery methods to optimize gene editing in agriculture.
纳米颗粒（NP）介导的基因传递为植物生物技术中的传统方法提供了一种有前景的替代方案，可促进基因转化，提高精度和效率。本文综述讨论了影响NP功效的关键因素，包括植物细胞壁组成、DNA/NP比例、暴露时间、载药量以及转化后评估。我们探讨了NP细胞毒性、转化效率和再生方面的挑战，同时解决环境影响和监管考量。我们强调了刺激响应性NP和可扩展传递方法在优化农业基因编辑方面的潜力。
