Rivlin Michal, Navon Gil
School of Chemistry, Tel Aviv University, Tel Aviv, Israel.
J Biomol NMR. 2018 Oct;72(1-2):93-103. doi: 10.1007/s10858-018-0209-y. Epub 2018 Sep 10.
3-O-Methyl-D-glucose (3OMG) was recently suggested as an agent to image tumors using chemical exchange saturation transfer (CEST) MRI. To characterize the properties of 3OMG in solution, the anomeric equilibrium and the mutarotation rates of 3OMG were studied by H and C NMR. This information is essential in designing the in vivo CEST experiments. At room temperature, the ratio of α and β 3OMG anomers at equilibrium was 1:1.4, and the time to reach 95% equilibrium was 6 h. The chemical exchange rates between the hydroxyl protons of 3OMG and water, measured by CEST and spin lock at pH 6.14 and a temperature of 4 °C, were in the range of 360-670 s.
3 - O - 甲基 - D - 葡萄糖（3OMG）最近被提议作为一种利用化学交换饱和转移（CEST）磁共振成像（MRI）对肿瘤进行成像的试剂。为了表征3OMG在溶液中的性质，通过氢核磁共振（H NMR）和碳核磁共振（C NMR）研究了3OMG的异头物平衡和变旋速率。这些信息对于设计体内CEST实验至关重要。在室温下，平衡时α和β 3OMG异头物的比例为1:1.4，达到95%平衡的时间为6小时。在pH 6.14和4℃温度下，通过CEST和自旋锁定测量的3OMG羟基质子与水之间的化学交换速率在360 - 670 s范围内。
