Vander Tuig J G, Kerner J, Crist K A, Romsos D R
Metabolism. 1986 Oct;35(10):960-6. doi: 10.1016/0026-0495(86)90062-4.
Rats with obesity-producing, hypothalamic knife cuts were fed a high fat diet and placed in the cold (2 degrees C) for six days starting 3, 11, or 24 days after surgery. Between surgery and cold exposure, knife-cut rats consumed 90% to 122% more energy and gained more weight (32 +/- 4, 112 +/- 5, and 241 +/- 9 g) than sham-operated rats (15 +/- 2, 34 +/- 2, and 58 +/- 3 g). When exposed to cold, sham-operated rats increased (22% to 30%) energy intake whereas knife-cut rats decreased (5% to 51%) intake. After 24 hours at 2 degrees C body temperatures of knife-cut rats were 1.2, 0.7, and 0.7 degrees less than those of control rats; body temperatures continued to decrease to 2.9, 3.0 and 2.5 degrees less than control rats after six days at 2 degrees C. Fasting for 12 hours at 2 degrees C caused a further reduction in body temperature to 4.9, 4.8, and 5.9 degrees less than in control rats. Cold exposure increased urinary excretion of norepinephrine and epinephrine (indicators of sympathoadrenal activity) in all rats. Guanosine diphosphate (GDP) binding to brown adipose tissue (BAT) mitochondria (an indicator of the thermogenic capacity of the tissue) was similar in cold-exposed, knife-cut, and sham-operated rats. Cold acclimation before hypothalamic knife-cut surgery prevented the cold-induced decrease in body temperatures of knife-cut rats.(ABSTRACT TRUNCATED AT 250 WORDS)
对患有肥胖症的大鼠进行下丘脑切断术后，喂食高脂饮食，并在术后3天、11天或24天开始置于寒冷环境（2摄氏度）中6天。在手术和暴露于寒冷环境之间，切断下丘脑的大鼠比假手术大鼠消耗的能量多90%至122%，体重增加更多（分别为32±4克、112±5克和241±9克，而假手术大鼠分别为15±2克、34±2克和58±3克）。暴露于寒冷环境时，假手术大鼠的能量摄入量增加（22%至30%），而切断下丘脑的大鼠摄入量减少（5%至51%）。在2摄氏度环境中24小时后，切断下丘脑的大鼠体温比对照大鼠低1.2摄氏度、0.7摄氏度和0.7摄氏度；在2摄氏度环境中6天后，体温继续下降，比对照大鼠低2.9摄氏度、3.0摄氏度和2.5摄氏度。在2摄氏度环境中禁食12小时导致体温进一步降低，比对照大鼠低4.9摄氏度、4.8摄氏度和5.9摄氏度。寒冷暴露使所有大鼠的去甲肾上腺素和肾上腺素尿排泄量增加（交感肾上腺活动指标）。在寒冷暴露的、切断下丘脑的和假手术的大鼠中，鸟苷二磷酸（GDP）与棕色脂肪组织（BAT）线粒体的结合（组织产热能力指标）相似。在下丘脑切断手术前进行冷适应可防止切断下丘脑的大鼠体温因寒冷而下降。（摘要截断于250字）
