Köster W, Braun V
Mol Gen Genet. 1986 Sep;204(3):435-42. doi: 10.1007/BF00331021.
The genes fhuA, fhuC and fhuD encode products involved in the transport of ferrichrome into cells of Escherichia coli. The nucleotide sequence of an additional locus contiguous to fhuD and formerly designated fhuB was determined. It contains an open reading frame for a polypeptide that consists of 659 amino acids. Expression of plasmids containing the fhuB region in an in vitro transcription/translation system and in E. coli minicells revealed a polypeptide with an electrophoretic mobility on SDS-polyacrylamide gels extremely dependent on the experimental conditions employed. This property could be explained by the very hydrophobic nature of the protein and it was concluded that the fhuB locus consists of a structural gene that encodes a membrane protein with a molecular weight of 82,181. The fhuB gene is required for all iron transport systems which operate via hydroxamate compounds. The order of the genes in the fhu operon is fhuA fhuC fhuD fhuB, and transcription proceeds from fhuA to fhuB.
基因fhuA、fhuC和fhuD编码参与将高铁色素转运到大肠杆菌细胞中的产物。测定了与fhuD相邻且先前命名为fhuB的一个额外基因座的核苷酸序列。它包含一个由659个氨基酸组成的多肽的开放阅读框。在体外转录/翻译系统和大肠杆菌微小细胞中含有fhuB区域的质粒的表达揭示了一种在SDS-聚丙烯酰胺凝胶上的电泳迁移率极其依赖所用实验条件的多肽。这种特性可以由该蛋白质极强的疏水性来解释，并且得出结论，fhuB基因座由一个编码分子量为82,181的膜蛋白的结构基因组成。fhuB基因对于所有通过异羟肟酸化合物运作的铁转运系统都是必需的。fhu操纵子中的基因顺序是fhuA、fhuC、fhuD、fhuB，转录从fhuA向fhuB进行。
