[雷公藤内生真菌枝孢霉属lgt - 3的化学成分]

[Chemical Constituents of the Endophytic Fungus Rhinocladiella sp. lgt-3 from Tripterygium wilfordii].

作者信息

Peng Cheng, Yang Zhong-duo

出版信息

Zhong Yao Cai. 2016 Aug;39(8):1775-8.

PMID:30204382

Abstract

OBJECTIVE

To study the chemical constituents and monoamine oxidase inhibitory activity of Rhinocladiella sp. lgt-3,which was an endophytic fungus isolated from Tripterygium wilfordii.

METHODS

Compounds were isolated from by various column chromatography. The structures were elucidated by various spectral data. The activity was tested by using 96-well micro-plated method.

RESULTS

10 compounds were isolated and identified as 3,4-dihydro-3,4,8-trihydroxy-1( 2H)-naphthalenone( 1),( 3R,4R)-4-hydroxymellein( 2),O-methylmellein( 3),4-( 2-hydroxybutynoxy) benzoic acid( 4),2,4-dihydroxy-6-(( R)-4-hydroxy-2-oxopentyl)-3-methylbenzaldehyde( 5),( Z)-N-( 4-hydroxystyryl) formamide( 6), aspterric acid( 7),bufotenin( 8),5-hydroxy-N,N-dimethyltryptamine-N-oxide( 9)and 5-methoxy-N,N-dimethyltryptamine( 10). Compounds 1,8 ~ 10 showed moderate anti-monoamine oxidase activity with IC50 values of 32,36,50,56 μmol/L, respectively.

CONCLUSION

Compounds 3 ~ 10 are isolated from Rhinocladiella genus for the first time and the anti-monoamine oxidase activity of compound 1 are reported for the first time.

摘要

目的

研究从雷公藤中分离得到的内生真菌 Rhinocladiella sp. lgt-3 的化学成分及单胺氧化酶抑制活性。

方法

采用多种柱色谱法分离化合物，通过各种光谱数据鉴定结构，用 96 孔微孔板法测试活性。

结果

分离鉴定出 10 种化合物，分别为 3,4-二氢-3,4,8-三羟基-1(2H)-萘酮(1)、(3R,4R)-4-羟基水合橙皮内酯(2)、O-甲基水合橙皮内酯(3)、4-(2-羟基丁炔氧基)苯甲酸(4)、2,4-二羟基-6-((R)-4-羟基-2-氧代戊基)-3-甲基苯甲醛(5)、(Z)-N-(4-羟基苯乙烯基)甲酰胺(6)、曲霉酸(7)、蟾蜍色胺(8)、5-羟基-N,N-二甲基色胺-N-氧化物(9)和 5-甲氧基-N,N-二甲基色胺(10)。化合物 1、8~10 显示出中等程度的抗单胺氧化酶活性，IC50 值分别为 32、36、50、56 μmol/L。