Debellé F, Sharma S B
Nucleic Acids Res. 1986 Sep 25;14(18):7453-72. doi: 10.1093/nar/14.18.7453.
A 6 kb DNA segment of the R. meliloti 2011 pSym megaplasmid, which contains genes controlling host specificity of root hair infection and of nodulation, was cloned and sequenced. The DNA sequence analysis, in conjunction with previous genetic data, allowed identification of four nod genes designated as E, F, G and H. nodH is divergently transcribed with respect to nodFE and nodG. A conserved nucleotide sequence was found around 200 bp upstream of the translation start of nodF, nodH and nodA. This sequence is also present upstream of common nodA and species specific nodF genes of other Rhizobium species. The predicted protein products of nodF and nodG show homology with acyl carrier protein and ribitol dehydrogenase, respectively. The nodH product contains a rare sequence of four contiguous proline residues. Comparison with the nod gene products of R. leguminosarum shows that species specific nodFE products are as well conserved as those of common nodABC and nodD genes.
根瘤菌2011共生大质粒上一段6 kb的DNA片段被克隆并测序，该片段包含控制根毛感染和结瘤宿主特异性的基因。结合先前的遗传数据进行DNA序列分析，鉴定出四个结瘤基因，分别命名为E、F、G和H。nodH与nodFE和nodG反向转录。在nodF、nodH和nodA翻译起始位点上游约200 bp处发现了一个保守的核苷酸序列。该序列也存在于其他根瘤菌属常见的nodA基因和物种特异性nodF基因的上游。预测的nodF和nodG蛋白产物分别与酰基载体蛋白和核糖醇脱氢酶具有同源性。nodH产物包含四个连续脯氨酸残基的罕见序列。与豌豆根瘤菌的结瘤基因产物比较表明，物种特异性的nodFE产物与常见的nodABC和nodD基因产物一样保守。
