Török I, Kondorosi E, Stepkowski T, Pósfai J, Kondorosi A
Nucleic Acids Res. 1984 Dec 21;12(24):9509-24. doi: 10.1093/nar/12.24.9509.
A Rhizobium meliloti DNA region, determining nodulation functions common in different Rhizobium species, has been delimited by directed Tn5 mutagenesis and its nucleotide sequence has been determined. The sequence data indicates three large open reading frames with the same polarity coding for three proteins of 196, 217 and 402 (or 426) amino acid residues, respectively. We suggest the existence of three nod genes on this region, which were designated as nodA, B and C, respectively. Comparison of the R. meliloti nodA, B, C nucleotide and amino acid sequences with those from R. leguminosarum, as reported in the accompanying paper, shows 69-72% homology, clearly demonstrating the high degree of conservation of common nod genes in these Rhizobium species.
通过定向Tn5诱变已界定了苜蓿根瘤菌(Rhizobium meliloti)中一个决定不同根瘤菌属中共有的结瘤功能的DNA区域,并测定了其核苷酸序列。序列数据表明有三个具有相同极性的大的开放阅读框,分别编码由196、217和402(或426)个氨基酸残基组成的三种蛋白质。我们认为该区域存在三个结瘤基因,分别命名为nodA、B和C。如随附论文中所报道的,将苜蓿根瘤菌的nodA、B、C核苷酸和氨基酸序列与豆科根瘤菌(R. leguminosarum)的相应序列进行比较,显示出69 - 72%的同源性,清楚地证明了这些根瘤菌属中共有结瘤基因的高度保守性。