Department of Marine Technology, School of Marine and Biological Engineering, Yancheng Institute of Technology, Yancheng, 224051, Province Jiangsu, China.
U.S. Department of Agriculture, Agricultural Research Service, Aquatic Animal Health Research Unit, 990 Wire Road, Auburn, AL, 36832, USA.
Fish Shellfish Immunol. 2018 Dec;83:140-147. doi: 10.1016/j.fsi.2018.09.015. Epub 2018 Sep 8.
Gibel carp (Carassius auratus gibelio), a major aquaculture species in China, has emerged in a seriously epizootic disease caused by Cyprinid herpesvirus 2 (CyHV-2). There are no effective methods to prevent or control this serious disease. Biofloc technology (BFT) can improve water quality, reduce pathogens introduction, enhance cultured species immunity and disease resistance. In this study, a 30-day experiment was conducted to investigate the effect of BFT on innate immune response and disease resistance of gibel carp against CyHV-2 infection. Gibel carp was cultured at different total suspended solid (TSS) concentrations of 10, 300, 600, 800 and 1000 mg L, which were named as groups BF0, BF300, BF600, BF800 and BF1000. Results showed that fish in groups BF600/800 had significantly higher weight gain (WG) and specific growth rate (SGR) than them in control group (BF0). The transcriptional levels of seven immune-related genes in BF300/600/800 groups, including myeloid-specific- peroxidase (MPO), keratin 8 (KRT 8), dual specificity phosphatase 1 (DUSP 1), interleukin-11 (IL-11), intelectin (ITLN), purine nucleoside phosphorylase 5α (PNP 5α) and c-type lysozyme (c-lys), were up-regulated significantly compared to BF0 group. Furthermore, cumulative mortality of gibel carp in BF600 group after being challenged with CyHV-2 reduced significantly. In vivo viral replication in kidney demonstrated that CyHV-2 load at 168 h post injection in BF600 group was significantly higher than that in BF0 group. In conclusion, BFT could improve growth, immune response and disease resistance of gibel carp, and the effect was related with TSS concentration. The optimal TSS concentration of 600-800 mg L was recommended in the present study.
黄河鲤（Carassius auratus gibelio）是中国主要的水产养殖品种之一，已出现由鲤鱼疱疹病毒 2 型（CyHV-2）引起的严重流行病。目前尚无有效方法来预防或控制这种严重疾病。生物絮团技术（BFT）可以改善水质，减少病原体的引入，增强养殖品种的免疫力和抗病能力。本研究进行了为期 30 天的实验，以研究 BFT 对黄河鲤先天免疫反应和对 CyHV-2 感染的抗病能力的影响。黄河鲤在总悬浮固体（TSS）浓度为 10、300、600、800 和 1000mg/L 的不同条件下进行养殖，分别命名为 BF0、BF300、BF600、BF800 和 BF1000 组。结果表明，BF600/800 组的鱼的体重增加（WG）和特定生长率（SGR）明显高于对照组（BF0）。BF300/600/800 组的 7 种免疫相关基因的转录水平，包括髓过氧化物酶（MPO）、角蛋白 8（KRT 8）、双特异性磷酸酶 1（DUSP 1）、白细胞介素 11（IL-11）、凝集素（ITLN）、嘌呤核苷磷酸化酶 5α（PNP 5α）和 C 型溶菌酶（c-lys），与 BF0 组相比均显著上调。此外，BF600 组黄河鲤在感染 CyHV-2 后的累计死亡率明显降低。体内肾脏中的病毒复制表明，BF600 组在注射后 168 小时的 CyHV-2 载量明显高于 BF0 组。综上所述，BFT 可以提高黄河鲤的生长、免疫反应和抗病能力，效果与 TSS 浓度有关。本研究推荐使用 600-800mg/L 的最佳 TSS 浓度。
