Division of Fish Disease, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences; The Key Laboratory of Yangtze River Basin's Aquatic Animal Diseases, the Ministry of Agriculture of China, Wuhan 430223, PR China.
Vet Microbiol. 2013 Sep 27;166(1-2):138-44. doi: 10.1016/j.vetmic.2013.05.025. Epub 2013 Jun 14.
An epizootic with severe mortality has emerged in cultured gibel carp, Carassius auratus gibelio, in China since 2009, and caused huge economic loss. The signs and epidemiology background of the disease were investigated. Parasite examination, bacteria and virus isolation were carried out for pathogen isolation. The causative pathogen was obtained and identified as Cyprinid herpesvirus 2 (CyHV-2) by experimental infection, electron microscopy, cell culture, PCR assay and sequence alignment, designated as CyHV-2-JSSY. Experimental infection proved the high virulence of CyHV-2-JSSY to healthy gibel carp. Electron microscopy revealed that the viral nucleocapsid was hexagonal in shape measuring 110-120 nm in diameter with a 170-200 nm envelope. The virus caused significant CPE in Koi-Fin cells at the early passages, but not beyond the fifth passages. Sequence alignment of the partial viral helicase gene (JX566884) showed that it shared 99-100% identity to the published sequences of other CyHV-2 isolates. This study represented the first isolation and identification of CyHV-2 in cultured gibel carp in China and laid a foundation for the further studies of the disease.
自 2009 年以来，中国养殖的黄河鲤鱼（Carassius auratus gibelio）爆发了一种死亡率很高的流行病，造成了巨大的经济损失。本研究调查了该病的症状和流行病学背景。进行寄生虫检查、细菌和病毒分离以进行病原体分离。通过实验感染、电子显微镜、细胞培养、PCR 检测和序列比对，获得并鉴定了致病病原体为鲤鱼疱疹病毒 2 型（CyHV-2），并将其命名为 CyHV-2-JSSY。实验感染证明 CyHV-2-JSSY 对健康鲤鱼具有高致病性。电子显微镜显示，病毒核衣壳呈六边形，直径为 110-120nm，包膜为 170-200nm。该病毒在早期传代的锦鲤细胞中引起明显的 CPE，但不能超过第五代。部分病毒解旋酶基因（JX566884）的序列比对表明，它与其他已发表的 CyHV-2 分离株的序列具有 99-100%的同源性。本研究代表了中国首次从养殖黄河鲤鱼中分离和鉴定 CyHV-2，为进一步研究该病奠定了基础。
