Department of Microbiology and Parasitology, Federal University of Rio Grande do Norte, Avenida Salgado Filho, BR 101, Campus Universitario, Lagoa Nova, Natal, RN 59078-900, Brazil.
Laboratory of Marine Algae, Department of Systematics and Ecology, Federal University of Paraiba, Joao Pessoa, PB 58051-900, Brazil.
Mar Drugs. 2018 Sep 7;16(9):318. doi: 10.3390/md16090318.
Caulerpin (CLP), an alkaloid from algae of the genus Caulerpa, has shown anti-inflammatory activity. Therefore, this study aimed to analyze the effect of CLP in the murine model of peritonitis and ulcerative colitis. Firstly, the mice were submitted to peritonitis to evaluate which dose of CLP (40, 4, or 0.4 mg/kg) could decrease the inflammatory infiltration in the peritoneum. The most effective doses were 40 and 4 mg/kg. Then, C57BL/6 mice were submitted to colitis development with 3% dextran sulfate sodium (DSS) and treated with CLP at doses of 40 and 4 mg/kg. The disease development was analyzed through the disease activity index (DAI); furthermore, colonic tissue samples were submitted to histological analysis, NFκB determination, and in vitro culture for cytokines assay. Therefore, CLP at 4 mg/kg presented the best results, triggering improvement of DAI and attenuating the colon shortening and damage. This dose was able to reduce the TNF-α, IFN-γ, IL-6, IL-17, and NFκB p65 levels, and increased the levels of IL-10 in the colon tissue. Thus, CLP mice treatment at a dose of 4 mg/kg showed promising results in ameliorating the damage observed in the ulcerative colitis.
钙泊三醇（CLP）是一种来自绿藻类的生物碱，具有抗炎活性。因此，本研究旨在分析 CLP 在腹膜炎和溃疡性结肠炎小鼠模型中的作用。首先，将小鼠进行腹膜炎实验，以评估 CLP（40、4 或 0.4mg/kg）的哪种剂量可以减少腹膜的炎症浸润。最有效的剂量为 40 和 4mg/kg。然后，用 3%葡聚糖硫酸钠（DSS）诱导 C57BL/6 小鼠发生结肠炎，并以 40 和 4mg/kg 的剂量给予 CLP 治疗。通过疾病活动指数（DAI）分析疾病的发展；此外，对结肠组织样本进行组织学分析、NFκB 测定和细胞因子体外培养分析。结果表明，4mg/kg 的 CLP 效果最佳，可改善 DAI，并减轻结肠缩短和损伤。该剂量可降低结肠组织中 TNF-α、IFN-γ、IL-6、IL-17 和 NFκB p65 的水平，并增加 IL-10 的水平。因此，4mg/kg 的 CLP 治疗剂量在改善溃疡性结肠炎观察到的损伤方面显示出良好的效果。
