全反式视黄醛刺激中性粒细胞释放超氧化物并激活磷脂酶C活性，而对蛋白激酶C无明显抑制作用。
all-trans-Retinal stimulates superoxide release and phospholipase C activity in neutrophils without significantly blocking protein kinase C.
作者信息
Lochner J E, Badwey J A, Horn W, Karnovsky M L
出版信息
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7673-7. doi: 10.1073/pnas.83.20.7673.
Abstract
all-trans-Retinal was previously shown to stimulate high levels of superoxide release by guinea pig neutrophils. When the cells, previously labeled with [3H]inositol, are treated with all-trans-retinal, they exhibit a decrease in the levels of [3H]inositol phospholipids and an increase in the accumulation of [3H]inositol phosphates. The maximal accumulation of inositol phosphates and the optimal rate of superoxide release occurred together at approximately 7 min after stimulation. The levels of [3H]inositol phosphates accumulated were comparable to those observed when the cells were stimulated with a chemotactic peptide. In direct measurements, using concentrations that stimulate intact cells maximally, all-trans-retinal was found not to inhibit protein kinase C from the cytosol of neutrophils significantly. This contrasts with the situation with this kinase obtained from other sources. These observations represent additional effects of vitamin A on cells.
摘要
全反式视黄醛先前已被证明可刺激豚鼠中性粒细胞释放高水平的超氧化物。当先前用[3H]肌醇标记的细胞用全反式视黄醛处理时，它们会表现出[3H]肌醇磷脂水平的降低以及[3H]肌醇磷酸积累的增加。肌醇磷酸的最大积累和超氧化物释放的最佳速率在刺激后约7分钟同时出现。积累的[3H]肌醇磷酸水平与用趋化肽刺激细胞时观察到的水平相当。在直接测量中，使用最大程度刺激完整细胞的浓度时，发现全反式视黄醛不会显著抑制中性粒细胞胞质溶胶中的蛋白激酶C。这与从其他来源获得的这种激酶的情况形成对比。这些观察结果代表了维生素A对细胞的其他作用。
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all-trans-Retinal stimulates superoxide release and phospholipase C activity in neutrophils without significantly blocking protein kinase C.全反式视黄醛刺激中性粒细胞释放超氧化物并激活磷脂酶C活性，而对蛋白激酶C无明显抑制作用。
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7673-7. doi: 10.1073/pnas.83.20.7673.
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Effect of retinol and retinoic acid on permeability, electrical resistance and phase transition of lipid bilayers.视黄醇和视黄酸对脂质双层膜通透性、电阻及相变的影响。
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Synergistic activation by collagen and 15-hydroxy-9 alpha,11 alpha-peroxidoprosta-5,13-dienoic acid (PGH2) of phosphatidylinositol metabolism and arachidonic acid release in human platelets.胶原蛋白与15-羟基-9α,11α-过氧前列腺素-5,13-二烯酸（PGH2）协同激活人血小板中磷脂酰肌醇代谢及花生四烯酸释放。
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