Ruminant Nutrition and Physiology Laboratory, College of Animal Science and Technology, Shandong Agricultural University, Taian, 271018, P.R. China.
Agriculture and Agri-Food Canada, Lethbridge Research Centre, Alberta, T1J 4B1, Canada.
Sci Rep. 2018 Sep 11;8(1):13574. doi: 10.1038/s41598-018-31845-0.
Serum proteins are sensitive with diseases in dairy cows, and some of them could be used as biomarkers for fatty liver. This study aimed to explore serum biomarkers for fatty liver in dairy cows. A total of 28 early lactating dairy cows were chosen from a commercial dairy herds, liver samples were collected for determining concentration of triacylglycerol (TAG), and serum samples were collected for measuring fibroblast growth factor-21 (FGF-21), adiponectin, Lipoprotein-associated phospholipase A2 (LP-PLA2), and hemoglobin (Hb). Dairy cows were divided into fatty liver (liver TAG > 5%, wet weight) and control group (liver TAG < 5%, wet weight). Concentration of FGF-21 was greater in fatty liver cows, while the concentration of LP-PLA2 and Hb was less. The concentration of FGF-21 and total Hb had strong correlation with the liver TAG as well as good prediction power (kappa value = 0.79 and 0.58, respectively). These results suggested that the serum concentration of FGF-21 and total Hb could be potentially used as fatty liver biomarkers in lactating dairy cows.
血清蛋白对奶牛疾病敏感，其中一些可作为脂肪肝的生物标志物。本研究旨在探索奶牛脂肪肝的血清生物标志物。从一个商业奶牛场选择了 28 头泌乳早期奶牛，采集肝脏样本以测定三酰甘油(TAG)浓度，采集血清样本以测定成纤维细胞生长因子-21 (FGF-21)、脂联素、脂蛋白相关磷脂酶 A2 (LP-PLA2)和血红蛋白 (Hb)。奶牛分为脂肪肝（肝 TAG>5%，湿重）和对照组（肝 TAG<5%，湿重）。脂肪肝奶牛的 FGF-21 浓度较高，而 LP-PLA2 和 Hb 的浓度较低。FGF-21 和总 Hb 的浓度与肝 TAG 具有很强的相关性，并且具有良好的预测能力（kappa 值分别为 0.79 和 0.58）。这些结果表明，血清 FGF-21 和总 Hb 的浓度可能可作为泌乳奶牛脂肪肝的生物标志物。
