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LncRNA XLOC_006390 作为 ceRNA 对抗 miR-331-3p 和 miR-338-3p，促进宫颈癌的发生和转移。

LncRNA XLOC_006390 facilitates cervical cancer tumorigenesis and metastasis as a ceRNA against miR-331-3p and miR-338-3p.

机构信息

Department of Obstetrics and Gynecology, Affiliated Hospital of Qingdao University, Qingdao, China.

出版信息

J Gynecol Oncol. 2018 Nov;29(6):e95. doi: 10.3802/jgo.2018.29.e95.

DOI:10.3802/jgo.2018.29.e95
PMID:30207103
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6189437/
Abstract

OBJECTIVE

Cervical cancer is one of the most common malignant tumors. Our previous results showed that long non-coding RNA (lncRNA) XLOC_006390 plays an important role in cervical cancer. In this study, we have explored the mechanism of action of lncRNA XLOC_006390.

METHODS

LncRNA XLOC_006390 was proposed to exercise its function as a competing endogenous RNA (ceRNA), and its potential targeted miRNAs was predicted through the database LncBase Predicted v.2. Two miRNAs, miR-331-3p, and miR-338-3p, were chosen for the study. Expression of miRNAs and lncRNA in cervical cancer cells and tissues was detected by reverse transcription polymerase chain reaction. To determine the correlation, silencing of XLOC_006390, over-expression of miR-331-3p, and miR-338-3p was performed in SiHa and Caski cell lines, respectively.

RESULTS

Based on the interactive effect between miRNA and lncRNA, miR-331-3p and miR-338-3p were significantly downregulated in cervical cancer cells and tissues, and their expression levels were negatively related to that of lncRNA. Our results also showed that the expression of miR-331-3p target gene , miR-338-3p target genes , was significantly downregulated when the XLOC_006390 was knocked down. Further, XLOC_006390 was found to facilitate cervical cancer tumorigenesis and metastasis by downregulating miR-331-3p and miR-338-3p expression.

CONCLUSION

Taken together, our study demonstrated that XLOC_006390 may serve as a ceRNA and reversely regulates the expression of miR-331-3p and miR-338-3p, thus facilitating cervical cancer tumorigenesis and metastasis.

摘要

目的

宫颈癌是最常见的恶性肿瘤之一。我们之前的研究结果表明，长链非编码 RNA（lncRNA）XLOC_006390 在宫颈癌中发挥重要作用。在本研究中，我们探讨了 lncRNA XLOC_006390 的作用机制。

方法

lncRNA XLOC_006390 被提出作为竞争性内源 RNA（ceRNA）发挥其功能，并通过数据库 LncBase Predicted v.2 预测其潜在的靶向 microRNA。选择了 microRNA-331-3p 和 microRNA-338-3p 进行研究。通过逆转录聚合酶链反应检测 microRNA 和 lncRNA 在宫颈癌细胞和组织中的表达。为了确定相关性，分别在 SiHa 和 Caski 细胞系中沉默 XLOC_006390、过表达 microRNA-331-3p 和 microRNA-338-3p。

结果

基于 microRNA 和 lncRNA 的相互作用，microRNA-331-3p 和 microRNA-338-3p 在宫颈癌细胞和组织中显著下调，其表达水平与 lncRNA 呈负相关。我们的结果还表明，当 XLOC_006390 被敲低时，miR-331-3p 靶基因和 miR-338-3p 靶基因的表达显著下调。此外，XLOC_006390 通过下调 miR-331-3p 和 miR-338-3p 的表达促进宫颈癌的发生和转移。

结论

综上所述，我们的研究表明，XLOC_006390 可能作为 ceRNA，反向调节 miR-331-3p 和 miR-338-3p 的表达，从而促进宫颈癌的发生和转移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/48cb82560dbd/jgo-29-e95-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/34dfc331a993/jgo-29-e95-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/219730d185bb/jgo-29-e95-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/dbdb1131a1de/jgo-29-e95-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/4c48652ba903/jgo-29-e95-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/93a143b3a12b/jgo-29-e95-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/48cb82560dbd/jgo-29-e95-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/34dfc331a993/jgo-29-e95-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/219730d185bb/jgo-29-e95-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/dbdb1131a1de/jgo-29-e95-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/4c48652ba903/jgo-29-e95-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/93a143b3a12b/jgo-29-e95-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b37/6189437/48cb82560dbd/jgo-29-e95-g006.jpg

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