Maleki Abbas, Ghafourian Sobhan, Taherikalani Morovat, Soroush Setareh
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Infect Disord Drug Targets. 2019;19(2):118-127. doi: 10.2174/1871526518666180911142806.
Nowadays, due to the biofilm formation among coagulase-negative staphylococci (CoNS), acquisition of antibiotic resistance and virulence genes could be noted. These organisms resemble other staphylococcus that harbor mecA gene, which encode methicillin resistance but the diversity in CoNS is higher than other species. Based on increasing antibiotic resistance in Staphylococcus haemolyticus, analyzing of phenotypic and genotypic biofilm formation, antibiotic resistance and genes involved in this process, SCCmec and ACME typing were the aims of the current study.
256 clinical CoNS isolates were collected that 49 isolates were identified as S. haemolyticus. For evaluation the antibiotic resistance patterns, disk agar diffusion method was applied, and also biofilm assay carried out among methicillin resistant S. haemolyticus and SCCmec and ACME typing.
The results of antibiotic susceptibility indicated that the highest resistance was found for cotrimoxazole and erythromycin (86.6%) and the frequency of blaA (96.5%) and msrA (79.3%) genes was much higher than others. Among MRSH isolates 58.6 % showed a weak biofilm phenotype and 41.4% demonstrated a moderate biofilm density. Also, among the biofilm correlated genes, IS 256 (79.31%) was the most frequent. The SCCmec typing of MRSH isolates indicated that the type V was dominant.
Our findings indicated that the correlation between high rates of existence Is 256 gene and high prevalence of weak biofilm phenotype was among MRSH isolates. The current study revealed that multiple antibiotic resistance existed in S. haemolyticus isolates that is a warning for public health.
如今，由于凝固酶阴性葡萄球菌（CoNS）中生物膜的形成，可以观察到抗生素耐药性和毒力基因的获得。这些微生物与其他携带mecA基因的葡萄球菌相似，mecA基因编码耐甲氧西林，但CoNS中的多样性高于其他物种。基于溶血葡萄球菌抗生素耐药性的增加，分析表型和基因型生物膜形成、抗生素耐药性以及参与此过程的基因、SCCmec和ACME分型是本研究的目的。
收集了256株临床CoNS分离株，其中49株被鉴定为溶血葡萄球菌。为评估抗生素耐药模式，采用纸片琼脂扩散法，并对耐甲氧西林溶血葡萄球菌进行生物膜检测以及SCCmec和ACME分型。
抗生素敏感性结果表明，对复方新诺明和红霉素的耐药性最高（86.6%），blaA（96.5%）和msrA（79.3%）基因的频率远高于其他基因。在耐甲氧西林溶血葡萄球菌分离株中，58.6%表现出弱生物膜表型，41.4%表现出中等生物膜密度。此外，在生物膜相关基因中，IS 256（79.31%）最为常见。耐甲氧西林溶血葡萄球菌分离株的SCCmec分型表明V型占主导。
我们的研究结果表明，在耐甲氧西林溶血葡萄球菌分离株中，IS 256基因的高存在率与弱生物膜表型的高流行率之间存在相关性。当前研究表明溶血葡萄球菌分离株中存在多重抗生素耐药性，这对公共卫生是一个警示。
