Health Shandong Collaborative Innovation Center for Major Social Risk Prediction and Management, School of Public Health and Management, Weifang Medical University, Weifang, 261053, People's Republic of China.
Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, National Institute of Parasitic Diseases, Shanghai, 200025, People's Republic of China.
Parasitol Res. 2020 Apr;119(4):1259-1269. doi: 10.1007/s00436-020-06622-6. Epub 2020 Feb 14.
To reveal the genetic diversity of Babesia microti and Theileria orientalis in Southwest China, we conducted a molecular survey of piroplasms in hard ticks in a China-Myanmar border county. Host infesting and questing ticks were collected from Tengchong County in 2013 and 2014. Piroplasm infection in ticks was detected by PCR, and then, phylogenetic analysis was conducted to study the genetic diversity of the pathogens identified in ticks. All in all, six piroplasm species comprising of B. microti; B. orientalis; a novel Babesia species designated Babesia sp. Tengchong, China; T. orientalis; T. luwenshuni; and an as yet undescribed piroplasmid species referred to as Piroplasmid sp. Tengchong, China, have been identified after screening goat- and cattle-attached ticks. In addition, B. bigemina has been identified by screening questing ticks. Phylogenetic analysis based on the 18S rRNA and partial β-tubulin gene revealed two novel potentially zoonotic genotypes designated B. microti Tengchong-Type A and B. The T. orientalis genotypes identified in the present study represent the seven known genotypes 1-5, 7, and N3 as revealed by phylogenetic analysis of 18S rRNA and MPSP genes. Importantly, an additional genotype designated N4 has also been identified in this study, which brings the number of recognized T. orientalis genotypes to a total of twelve. Thus, besides the two novel species, Babesia sp. Tengchong, China, closely related to Babesia species isolated from yak and Piroplasmid sp. Tengchong, China, our study demonstrates that additional novel B. microti and T. orientalis genotypes exist in Southwest China.
为了揭示中缅边境西南地区微小巴贝斯虫和东方泰勒虫的遗传多样性，我们对该边境县的硬蜱中的梨形虫进行了分子调查。2013 年和 2014 年，我们从腾冲县采集了寄生和刺探宿主的蜱。通过 PCR 检测蜱中的梨形虫感染情况，然后进行系统发育分析，研究在蜱中鉴定出的病原体的遗传多样性。总共从山羊和牛附着的蜱中筛选出了六种梨形虫，包括微小巴贝斯虫；东方泰勒虫；一种新的巴贝斯虫，命名为中国腾冲巴贝斯虫；东方泰勒虫；卢文西尼泰勒虫；和一种尚未描述的被称为中国腾冲未定种的梨形虫。此外，通过筛选刺探的蜱还鉴定出双芽巴贝斯虫。基于 18S rRNA 和部分β-微管蛋白基因的系统发育分析揭示了两种新的潜在人畜共患病基因型，分别命名为腾冲微小巴贝斯虫 A 型和 B 型。本研究中鉴定的东方泰勒虫基因型代表了七个已知的基因型 1-5、7 和 N3，这是通过 18S rRNA 和 MPSP 基因的系统发育分析揭示的。重要的是，在本研究中还鉴定出了一个额外的基因型 N4，使已知的东方泰勒虫基因型总数达到 12 个。因此，除了两种新的微小巴贝斯虫和东方泰勒虫外，中国腾冲的巴贝斯虫与从牦牛中分离出的巴贝斯虫密切相关，中国腾冲的未定种与东方泰勒虫密切相关，我们的研究表明，在中国西南地区还存在其他新的微小巴贝斯虫和东方泰勒虫基因型。
