Materials Science and Engineering, University of Michigan, 2300 Hayward St., Ann Arbor, MI 48109-2136, USA.
Research Institute of Advanced Materials, College of Engineering, Seoul National University, 028826, South Korea.
Analyst. 2018 Oct 7;143(19):4623-4629. doi: 10.1039/c8an01097h. Epub 2018 Sep 12.
We developed a paper-based analytical device (μPAD) combined with self-signaling polydiacetylene (PDA) liposomes for convenient visual neomycin detection. The simple dot array type of μPAD was fabricated by the wax printing technique, and the PDA liposomes in the aqueous solution were facilely immobilized onto the hydrophilic dot region of the paper substrate. We found that, when the PDA liposomes were inserted to the paper matrix, the stability of the PDA liposomes can be significantly enhanced by adding a hydrophilic reagent such as polyvinyl alcohol and glycerol to the liposome solution. In particular, polyvinyl alcohol (PVA) provides the best stabilization among the various hydrophilic reagents tested in this contribution, and the enhanced stability sharply increased the sensitivity of the PDA liposomes in the paper matrix. Based on the above results, we successfully detected neomycin through both naked-eye observation and fluorescence measurement of PDA signals. The detection limit was 1 ppm and was selective to non-aminoglycoside antibiotics.
我们开发了一种基于纸张的分析装置（μPAD），结合了自信号多乙酰（PDA）脂质体，用于方便的视觉新霉素检测。μPAD 的简单点阵列类型是通过蜡印技术制造的，并且 PDA 脂质体在水溶液中可轻易地固定在纸基底的亲水点区域上。我们发现，当 PDA 脂质体插入纸基质中时，通过向脂质体溶液中添加亲水剂如聚乙烯醇和甘油，可以显著增强 PDA 脂质体的稳定性。特别是，聚乙烯醇（PVA）在本研究中测试的各种亲水剂中提供了最佳的稳定性，增强的稳定性极大地提高了 PDA 脂质体在纸基质中的灵敏度。基于上述结果，我们成功地通过 PDA 信号的肉眼观察和荧光测量来检测新霉素。检测限为 1ppm，对非氨基糖苷类抗生素具有选择性。
