Kim Changheon, Hong Changgi, Lee Kangwon
Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea.
Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea.
Biosens Bioelectron. 2021 Jun 1;181:113120. doi: 10.1016/j.bios.2021.113120. Epub 2021 Mar 2.
Polydiacetylene (PDA) is a versatile polymer that has been studied in numerous fields because of its unique optical properties derived from alternating multiple bonds in the polymer backbone. The conjugated structure in the polymer backbone enables PDA to possess the ability of blue-red colorimetric transition when π-π interactions in the PDA backbone chain are disturbed by the external environment. The chromatic property of PDA disturbed by external stimuli can also emit fluorescence in the red region. Owing to the unique characteristics of PDA, it has been widely studied in facile and label-free sensing applications based on colorimetric or fluorescence signals for several decades. Among the various PDA structures, membrane structures assembled by amphiphilic molecules are widely used as a versatile platform because facile modification of the synthetic membrane provides extensive applications, such as receptor-ligand interactions, resulting in potent biosensors. To use PDA as a sensory material, several methods have been studied to endow the specificity to PDA molecules and to amplify the signal from PDA supramolecules. This is because selective and sensitive detection of target materials is required at an appropriate level corresponding to each material for applicable sensor applications. This review focuses on factors that affect the sensitivity of PDA composites and several strategies to enhance the sensitivity of the PDA sensor to various structures. Owing to these strategies, the PDA sensor system has achieved a higher level of sensitivity and selectivity, enabling it to detect multiple target materials for a full field of application.
聚二乙炔（PDA）是一种用途广泛的聚合物，由于其聚合物主链中交替的多重键所衍生的独特光学性质，已在众多领域得到研究。聚合物主链中的共轭结构使PDA在其主链链中的π-π相互作用受到外部环境干扰时，具备蓝-红比色转变的能力。受外部刺激干扰的PDA的显色特性在红色区域也能发出荧光。由于PDA的独特特性，几十年来它一直在基于比色或荧光信号的简便且无标记传感应用中得到广泛研究。在各种PDA结构中，由两亲分子组装而成的膜结构被广泛用作通用平台，因为合成膜的简便修饰提供了广泛的应用，如受体-配体相互作用，从而产生高效的生物传感器。为了将PDA用作传感材料，人们研究了几种方法来赋予PDA分子特异性并放大来自PDA超分子的信号。这是因为对于适用的传感器应用，需要在与每种材料相对应的适当水平上对目标材料进行选择性和灵敏的检测。本综述重点关注影响PDA复合材料灵敏度的因素以及提高PDA传感器对各种结构灵敏度的几种策略。由于这些策略，PDA传感器系统已实现了更高水平的灵敏度和选择性，使其能够检测多种目标材料以用于整个应用领域。
