Department of Dentistry and Oral Health, Faculty of Health, Aarhus University, Aarhus, Denmark.
Department of Physics and Astronomy, Interdisciplinary Nanoscience Center (iNANO), Faculty of Science and Technology, Aarhus University, Aarhus, Denmark.
J Periodontal Res. 2018 Dec;53(6):1020-1028. doi: 10.1111/jre.12601. Epub 2018 Sep 12.
Strontium (Sr) enhances osteogenic differentiation of certain multipotent cells. Periodontal ligament cells (PDLCs) are known to be multipotent, and Sr might be useful in periodontal bone tissue engineering. This study investigates the effect of high concentration of Sr on the proliferation and osteogenic behavior of PDLCs in vitro.
Primary human PDLCs were cultured in MEM + 10% FBS without (Ctrl) or with Sr in four diverse concentrations: Sr1, 11.3 × 10 mg/L, human serum physiological level; Sr2, 13 mg/L, typical human serum level after strontium ranelate treatment; Sr3, 130 mg/L, and Sr4, 360 mg/L. The spreading area (2, 4, 6, 24 hours), proliferation rate (1, 3, 7 days), osteogenic behavior (alkaline phosphatase - ALP activity, 7 and 14 days; expression of osteogenic genes, ALP, Runt-related transcription factor 2 - RUNX2, osteopontin - OPN, osteocalcin - OCN, and osteoprotegerin -OPG, 1, 3, 7, 14, 21 days), and formation of mineralized nodules (14 and 21 days) of the PDLCs were assessed. Data were compared group- and period-wise using ANOVA tests.
Periodontal ligament cells cultured with Sr4 showed increased spreading area (after 4 hours), proliferation rate (from 3 days), and OCN and OPN (from 7 days) gene expression as compared to Ctrl, Sr1, Sr2, and Sr3. Sr4 also led to lower ALP activity (from 7 days), ALP (from 3 days), and RUNX2 (at 7 and 14 days) gene expression, together with more evident formation of mineralized nodules, compared to Ctrl, Sr1, Sr2, and Sr3.
Periodontal ligament cells responded to Sr4 with increased cellular proliferation and osteogenic behavior in vitro.
锶（Sr）可增强某些多能细胞的成骨分化。已知牙周韧带细胞（PDLCs）是多能的，Sr 可能在牙周骨组织工程中有用。本研究旨在探讨高浓度 Sr 对体外 PDLC 增殖和成骨行为的影响。
原代人 PDLCs 在 MEM+10%FBS 中培养（对照）或在 4 种不同浓度的 Sr 中培养：Sr1，11.3×10mg/L，人血清生理水平；Sr2，13mg/L，雷奈酸锶治疗后典型的人血清水平；Sr3，130mg/L，Sr4，360mg/L。评估 PDLCs 的铺展面积（2、4、6、24 小时）、增殖率（1、3、7 天）、成骨行为（碱性磷酸酶-ALP 活性，7 和 14 天；成骨基因表达，ALP、成 Runt 相关转录因子 2-RUNX2、骨桥蛋白-OPN、骨钙素-OCN、骨保护素-OPG，1、3、7、14、21 天）和矿化结节的形成（14 和 21 天）。数据按组和时间进行方差分析。
与对照、Sr1、Sr2 和 Sr3 相比，培养在 Sr4 中的 PDLCs 的铺展面积（4 小时后）、增殖率（3 天）和 OCN 和 OPN（7 天）基因表达增加。Sr4 还导致 ALP 活性（7 天）、ALP（3 天）和 RUNX2（7 和 14 天）基因表达降低，同时与对照、Sr1、Sr2 和 Sr3 相比，矿化结节的形成更加明显。
牙周韧带细胞对 Sr4 反应表现为体外细胞增殖和成骨行为增强。
