Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
Biochem Biophys Res Commun. 2020 Jul 12;528(1):160-167. doi: 10.1016/j.bbrc.2020.05.097. Epub 2020 May 26.
Periodontal disease is the most common chronic disease of the oral and maxillofacial region, causing alveolar bone loss and ultimate loss of tooth. The purpose of treatment of periodontal disease is to promote the regeneration of periodontal tissue, including alveolar bone, and implantation of fixtures to replace the missing tooth as a result of advanced periodontal disease also requires alveolar bone regeneration. Methylsulfonylmethane (MSM) is a sulfur compound with well-known anti-inflammatory effects but its effects on bone regeneration are unknown. In this study, we investigated the effects of MSM on osteogenic differentiation of human PDLSCs (hPDLSCs) in vitro and in vivo. Our results demonstrate that MSM not only promotes the proliferation but also promotes osteogenic differentiation of hPDLSCs. MSM increased the expression levels of osteogenic specific markers that ALP, OPN, OCN, Runx2, and OSX. Smad2/3 signaling pathway was reinforced by MSM. Runx2, which downstream of Smad pathway, was expressed in accordance. Consistent with in vitro results, in vivo calvarial defect model and transplantation model revealed that MSM induces hPDLSCs to differentiate into osteoblast, which express ALP, OPN and OCN highly and enhance bone formation. These results suggest that MSM promotes osteogenic differentiation and bone formation of hPDLSCs, and Smad2/3 / Runx2 / OSX / OPN may play critical roles in the MSM-induced osteogenic differentiation. Thus, MSM combined with hPDLSCs may be a good candidate for future clinical applications in alveolar bone regeneration and can be used for graft material in reconstructive dentistry.
牙周病是口腔颌面部最常见的慢性疾病，导致牙槽骨丧失，最终导致牙齿脱落。牙周病治疗的目的是促进牙周组织的再生，包括牙槽骨，而由于牙周病进展导致缺失的牙齿也需要牙槽骨再生。甲磺酰甲烷（MSM）是一种具有已知抗炎作用的硫化合物，但它对骨再生的影响尚不清楚。在这项研究中，我们研究了 MSM 对体外和体内人牙周膜干细胞（hPDLSCs）成骨分化的影响。我们的结果表明，MSM 不仅促进 hPDLSCs 的增殖，还促进其成骨分化。MSM 增加了碱性磷酸酶（ALP）、骨桥蛋白（OPN）、骨钙素（OCN）、Runx2 和 OSX 等成骨特异性标志物的表达水平。MSM 增强了 Smad2/3 信号通路。Smad 通路的下游基因 Runx2 也随之表达。与体外结果一致，体内颅骨缺损模型和移植模型表明，MSM 诱导 hPDLSCs 分化为成骨细胞，这些成骨细胞高度表达 ALP、OPN 和 OCN，并增强骨形成。这些结果表明，MSM 促进 hPDLSCs 的成骨分化和骨形成，Smad2/3/Runx2/OSX/OPN 可能在 MSM 诱导的成骨分化中发挥关键作用。因此，MSM 联合 hPDLSCs 可能是未来牙槽骨再生临床应用的良好候选物，可作为口腔修复领域中骨移植材料。
