Abdalla Mohamed Mahmoud, Lung Christie Y K, Bijle Mohammed Nadeem, Yiu Cynthia Kar Yung
Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
Dental Biomaterials, Faculty of Dental Medicine, Al-Azhar University, Cairo 11651, Egypt.
Materials (Basel). 2022 Aug 25;15(17):5854. doi: 10.3390/ma15175854.
The development of biomaterials that exhibit profound bioactivity and stimulate stem cell differentiation is imperative for the success and prognosis of vital pulp therapies. The objectives were to (1) synthesize calcium strontium silicate (CSR) ceramic through the sol−gel process (2) investigate its physicochemical properties, bioactivity, cytocompatibility, and its stimulatory effect on the differentiation of human dental pulp stem cells (HDPSC). Calcium silicate (CS) and calcium strontium silicate (CSR) were synthesized by the sol−gel method and characterized by x-ray diffraction (XRD). Setting time, compressive strength, and pH were measured. The in vitro apatite formation was evaluated by SEM-EDX and FTIR. The NIH/3T3 cell viability was assessed using an MTT assay. The differentiation of HDPSC was evaluated using alkaline phosphatase activity (ALP), and Alizarin red staining (ARS). Ion release of Ca, Sr, and Si was measured using inductive coupled plasma optical emission spectroscopy (ICP-OES). XRD showed the synthesis of (CaSrSiO4). The initial and final setting times were significantly shorter in CSR (5 ± 0.75 min, 29 ± 1.9 min) than in CS (8 ± 0.77 min, 31 ± 1.39 min), respectively (p < 0.05). No significant difference in compressive strength was found between CS and CSR (p > 0.05). CSR demonstrated higher apatite formation and cell viability than CS. The ALP activity was significantly higher in CSR 1.16 ± 0.12 than CS 0.92 ± 0.15 after 14 d of culture (p < 0.05). ARS showed higher mineralization in CSR than CS after 14 and 21 d culture times. CSR revealed enhanced differentiation of HDPSC, physicochemical properties, and bioactivity compared to CS.
开发具有深刻生物活性并能刺激干细胞分化的生物材料对于牙髓治疗的成功和预后至关重要。本研究的目的是：（1）通过溶胶 - 凝胶法合成硅酸钙锶（CSR）陶瓷；（2）研究其物理化学性质、生物活性、细胞相容性以及对人牙髓干细胞（HDPSC）分化的刺激作用。采用溶胶 - 凝胶法合成硅酸钙（CS）和硅酸钙锶（CSR），并通过X射线衍射（XRD）进行表征。测量凝固时间、抗压强度和pH值。通过扫描电子显微镜 - 能谱仪（SEM - EDX）和傅里叶变换红外光谱（FTIR）评估体外磷灰石形成情况。使用MTT法评估NIH / 3T3细胞活力。通过碱性磷酸酶活性（ALP）和茜素红染色（ARS）评估HDPSC的分化情况。使用电感耦合等离子体发射光谱仪（ICP - OES）测量Ca、Sr和Si的离子释放量。XRD显示合成了（CaSrSiO4）。CSR的初始和最终凝固时间（分别为5±0.75分钟和29±1.9分钟）明显短于CS（分别为8±0.77分钟和31±1.39分钟）（p < 0.05）。CS和CSR之间的抗压强度未发现显著差异（p > 0.05）。与CS相比，CSR表现出更高的磷灰石形成和细胞活力。培养14天后，CSR的ALP活性（1.16±0.12）明显高于CS（0.92±0.15）（p < 0.05）。在培养14天和21天后，ARS显示CSR中的矿化程度高于CS。与CS相比，CSR显示出HDPSC分化增强、物理化学性质和生物活性增强。
