Cai Xuan, Qiu Wen, Tian En-wei, Zhang Hong-wei, Ye Hao-ting, Chao Zhi
Zhong Yao Cai. 2016 Sep;39(9):1956-9.
To identify the original species of fish maw sold in Guangzhou market by DNA barcoding technology.
Mitochondrial cytochrome C subunit I (CO I) gene fragment of eleven fish maw samples were amplified and sequenced with the self-designed primers. UPGMA phylogenetic tree were constructed for clustering analysis. The species origin of each sample was identified with the identification engine provided in the Barcode of Life Data Systems (BOLD).
The self-designed primers were effective in fish maw CO I amplification and sequencing, with success rates both of 100%. BOLD identification and UPGMA clustering analysis indicated the fish maw samples were derived from five fish species of three families.
DNA barcoding combined with BOLD identification system can accurately identify the species origin of commercial fish maw.
运用DNA条形码技术鉴定广州市场上销售的鱼鳔的原始物种。
采用自行设计的引物对11份鱼鳔样本的线粒体细胞色素C亚基I（CO I）基因片段进行扩增和测序。构建UPGMA系统发育树进行聚类分析。利用生命条形码数据系统（BOLD）提供的鉴定引擎鉴定每个样本的物种来源。
自行设计的引物对鱼鳔CO I的扩增和测序有效，成功率均为100%。BOLD鉴定和UPGMA聚类分析表明，鱼鳔样本来自3个科的5种鱼类。
DNA条形码结合BOLD鉴定系统能够准确鉴定市售鱼鳔的物种来源。
