Arami Shinichiro, Sato Megumi, Futo Satoshi
Fasmac Co., Ltd., Atsugi-shi, Kanagawa.
Shokuhin Eiseigaku Zasshi. 2011;52(3):205-10. doi: 10.3358/shokueishi.52.205.
DNA barcoding is a species identification technique, which uses a very short DNA sequence from a region of approximately 650 base-pairs in the 5'-end of the mitochondrial cytochrome c oxidase subunit I gene as a marker to identify species of mammals and fishes. The applicability of DNA barcoding for identification of fish species consumed in Japan was studied. Among thirty-one fresh or processed fishes were obtained from the market, two samples could not be identified due to lack of data in the Barcode of Life Data (BOLD) database. However, BLAST-search of 16S rRNA genes in the National Center for Biotechnology Information (NCBI) database and the PCR-RFLP method published by the Food and Agricultural Materials Inspection Center (FAMIC) were found to be applicable to identify these 2 fishes. The results show that the DNA barcoding technique is potentially useful as a tool for confirming the proper labeling of fish species in the Japanese market.
DNA条形码是一种物种鉴定技术，它使用线粒体细胞色素c氧化酶亚基I基因5'端约650个碱基对区域的一段非常短的DNA序列作为标记，来鉴定哺乳动物和鱼类的物种。研究了DNA条形码在鉴定日本消费的鱼类物种方面的适用性。从市场上获取的31种新鲜或加工鱼类中，有两个样本由于生命条形码数据（BOLD）数据库中缺乏数据而无法鉴定。然而，发现美国国立生物技术信息中心（NCBI）数据库中16S rRNA基因的BLAST搜索以及食品和农业材料检验中心（FAMIC）公布的PCR-RFLP方法适用于鉴定这两种鱼类。结果表明，DNA条形码技术作为确认日本市场鱼类物种正确标签的工具具有潜在用途。
