Department of Prosthodontics, Balgat Oral and Dental Health Center, Ankara, Turkey.
Department of Biomaterials, University of Health Sciences, Istanbul, Turkey.
Clin Implant Dent Relat Res. 2018 Dec;20(6):1022-1029. doi: 10.1111/cid.12665. Epub 2018 Sep 12.
For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms. Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al O ) and zirconia (ZrO ) implant systems are widely used in the area. However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.
The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.
The substrates were coated via solution casting technique. Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment. To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used. The phases and bonds characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis. The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.
It was observed that both substrates were successfully coated with chitosan and the aging process did not significantly affect the integrity of the coating. The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.
Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.
为了长期成功地使用牙种植体，维持周围软组织屏障的健康至关重要，因为它可以保护骨-种植体界面免受微生物的侵害。尽管基于钛及其合金的种植体仍然主导着牙科种植体市场，但氧化铝（Al2O3）和氧化锆（ZrO2）种植体系统在该领域得到了广泛应用。然而，它们在黏膜下区域提供了光滑且惰性的表面，与周围组织的整合效果较差。
本研究的主要目的是研究壳聚糖涂覆的氧化铝和氧化锆表面的表面特性和生物相容性。
通过溶液浇铸技术对基底进行涂覆。此外，还应用热循环设备对涂覆材料进行老化处理，以模拟口腔环境。使用扫描电子显微镜和能量色散 X 射线光谱仪来定义未处理、壳聚糖涂覆和壳聚糖涂覆老化样品的表面形貌和化学组成。使用傅里叶变换红外光谱和 X 射线衍射分析来确定相和键。用人牙龈成纤维细胞通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑盐测定法来评估细胞相容性。
观察到两种基底都成功地涂覆了壳聚糖，而且老化过程并没有显著影响涂层的完整性。两种壳聚糖涂覆表面都显示出人牙龈成纤维细胞的附着和增殖良好。
在氧化锆和氧化铝表面涂覆壳聚糖是一种有效的表面改性方法，可以提高这些材料在体外的生物相容性和生物活性。
