Du Renjia, Jiao Shengyin, Dai Yue, An Jianbo, Lv Jia, Yan Xiaoni, Wang Juan, Han Bei
School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an, China.
Institute of Food and Agriculture, China National Institute of Standardization, Beijing, China.
Front Microbiol. 2018 Aug 28;9:2006. doi: 10.3389/fmicb.2018.02006. eCollection 2018.
Growth retardation of calves is defined as a symptom of impaired growth and development, probably due to growth hormone disorder as well as natural and environmental factors in livestock. The growth-promoting effects of probiotics were determined in 50 growth-retarded growth calves. They were supplied with C-1 (Ba, 4 × 10CFU/d, = 16), (Bs, 4 × 10CFU/d, = 18), and negative control (NC, = 16) for 30 days. Pre- and post-intervention, the growth performance (weight gain rate, feed intake and feed conversion rate) was analyzed, the serum GH, IGH-1 and immunoglobulin levels were assayed, and the fecal microbiota was detected. Calves in Ba and Bs groups demonstrated increased body weight gain, feed intake and GH/IGF-1 levels, as well as a more efficient feed conversion rate, compared with NC group ( < 0.05). Additionally, the abundances of bacteria contributing to the production of energy and SCFAs (short chain fatty acids), including , , and were increased compared with NC group ( < 0.05, < 0.1); and the suspected pathogens, which included and were decreased ( < 0.05, < 0.1) in both the Bs and Ba groups. , which is involved in the intestinal mucosal immune response, was increased in Bs group after intervention ( < 0.05, < 0.1), but exhibited no obvious difference in Ba group. The increased bacterial genera in Ba group were and ( < 0.05, < 0.1). These results indicate that the probiotics and exhibited similar therapeutic potential in terms of growth performance by regulating hormones, and improving the intestinal and rumen development in growth-retarded animals.
犊牛生长发育迟缓被定义为生长和发育受损的一种症状，这可能是由于生长激素紊乱以及家畜的自然和环境因素所致。在50头生长发育迟缓的犊牛中测定了益生菌的促生长作用。给它们分别提供C-1（芽孢杆菌属，4×10CFU/天，n = 16）、双歧杆菌属（4×10CFU/天，n = 18）和阴性对照（NC，n = 16），持续30天。在干预前后，分析生长性能（体重增加率、采食量和饲料转化率），测定血清生长激素（GH）、胰岛素样生长因子-1（IGF-1）和免疫球蛋白水平，并检测粪便微生物群。与NC组相比，芽孢杆菌属和双歧杆菌属组的犊牛体重增加、采食量和GH/IGF-1水平升高，饲料转化率更高（P < 0.05）。此外，与NC组相比，有助于产生能量和短链脂肪酸（SCFAs）的细菌丰度增加，包括丁酸弧菌属、瘤胃球菌属和粪杆菌属（P < 0.05，P < 0.1）；而包括大肠杆菌和沙门氏菌在内的疑似病原体在双歧杆菌属和芽孢杆菌属组中均减少（P < 0.05，P < 0.1）。参与肠道黏膜免疫反应的双歧杆菌属在干预后双歧杆菌属组中增加（P < 0.05，P < 0.1），但在芽孢杆菌属组中无明显差异。芽孢杆菌属组中增加的细菌属为理研菌属和颤螺菌属（P < 0.05，P < 0.1）。这些结果表明，益生菌芽孢杆菌属和双歧杆菌属通过调节激素以及改善生长发育迟缓动物的肠道和瘤胃发育，在生长性能方面表现出相似的治疗潜力。
