Bawdon R E, Madsen P O
Antimicrob Agents Chemother. 1986 Aug;30(2):231-3. doi: 10.1128/AAC.30.2.231.
A reverse-phase high-pressure liquid chromatography method for the quantitation of sulbactam in plasma, urine, and tissue is described. The assay used the formation of an imidazole derivative followed by extraction with acetonitrile and dichloromethane and used UV absorbance for detection. The mobile phase consisted of acetonitrile, tetrabutylammonium hydroxide, and phosphate buffer. The assay was linear from 100 micrograms/ml (g of tissue) to 1 microgram/ml (g). Within- and between-batch recovery was greater than 90%. The coefficient of variation was generally less than 15%. There were no interfering peaks in the quantitation of sulbactam.
描述了一种用于定量测定血浆、尿液和组织中舒巴坦的反相高压液相色谱法。该测定法利用咪唑衍生物的形成，随后用乙腈和二氯甲烷萃取，并采用紫外吸光度进行检测。流动相由乙腈、氢氧化四丁基铵和磷酸盐缓冲液组成。该测定法在100微克/毫升（组织克数）至1微克/毫升（克）范围内呈线性。批内和批间回收率均大于90%。变异系数一般小于15%。在舒巴坦的定量测定中没有干扰峰。
