Evaluation of B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis.

BACKGROUND

One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of in the placental tissues of pregnant women with acute toxoplasmosis.

MATERIALS AND METHODS

The study was a cross-sectional study. Serum samples of pregnant women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for immunoglobulin G (IgG) antibodies against by enzyme-linked immunosorbent assay. Then, polymerase chain reaction was used to identify the specific B1 gene of in IgG seropositive women. The placental tissues of the pregnant women with positive serum B1 gene examined for this gene. Anti- immunoglobulin M (IgM) was performed on the umbilical cord and neonate blood.

RESULTS

Anti- IgG was detected in 167 out of 653 (25.6%) pregnant women. B1 gene was identified in 36 out of 167 (21.6%) of IgG seropositive women. After delivery, the B1 gene was evaluated in 15 out of 36 (41.7%) patients' placental tissues, 13 of which were positive for this gene (86.7%). Anti- IgM was detected neither in any umbilical cord nor in neonatal blood samples. All newborns, with the exception of one case, were born with normal birth weight and in term birth.

CONCLUSION

The B1 gene was detected in 86.7% of the placental tissue of women who were involved in acute toxoplasmosis during pregnancy.