螯合剂对脑果糖-1,6-二磷酸酶活性的修饰：5'-AMP敏感性的“诱导”
Modification of brain fructose-1,6-bisphosphatase activity by chelators: "induction" of 5'-AMP sensitivity.
作者信息
Chattoraj S, Majumder A L
出版信息
Biochem Biophys Res Commun. 1986 Sep 14;139(2):571-80. doi: 10.1016/s0006-291x(86)80029-8.
Brain fructose-1,6-bisphosphatase (EC 3.1.3.11) from various sources are ordinarily insensitive to 5'-AMP. In addition to stimulation and conferring a "neutral" behaviour, prior treatment with histidine, EDTA or imidazole renders the brain enzyme sensitive to 5'-AMP. The histidine treated enzyme(s) bind to Blue-Sepharose CL-6B column and are specifically eluted by 5'-AMP in contrast to the untreated enzyme(s) which do not bind to the affinity column at all. The histidine effect in inducing 5'-AMP sensitivity was abolished by treatment of the native enzyme by subtilisin or by a number of divalent cations including Zn++.
来自不同来源的脑果糖-1,6-二磷酸酶（EC 3.1.3.11）通常对5'-AMP不敏感。除了具有刺激作用并表现出“中性”行为外，用组氨酸、EDTA或咪唑预先处理会使脑酶对5'-AMP敏感。与根本不与亲和柱结合的未处理酶相比，经组氨酸处理的酶会结合到Blue-Sepharose CL-6B柱上，并被5'-AMP特异性洗脱。通过枯草杆菌蛋白酶或包括Zn++在内的多种二价阳离子处理天然酶，可消除组氨酸诱导5'-AMP敏感性的作用。
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