关键基因启动子区域 H3K27ac 和 H3K27me3 的相互变化与子宫内膜蜕膜化有关。
Reciprocal changes of H3K27ac and H3K27me3 at the promoter regions of the critical genes for endometrial decidualization.
机构信息
Department of Maternal-Fetal Biology, National Research Institute for Child Health & Development, Tokyo 157-8535, Japan.
Department of Obstetrics & Gynecology, Faculty of Medicine, Juntendo University, Tokyo 113-8431, Japan.
出版信息
Epigenomics. 2018 Sep;10(9):1243-1257. doi: 10.2217/epi-2018-0006. Epub 2018 Sep 13.
AIM
Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome and epigenome profiles for primary endometrial stromal cells (ESCs) and in vitro decidualized cells.
MATERIALS & METHODS: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone.
RESULTS
Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization. Subsets of up- and down-regulated genes upon decidualization were associated with reciprocal changes of H3K27ac and H3K27me3 modifications at their promoter region, and were enriched with genes essential for decidualization such as WNT4, ZBTB16, PROK1 and GREB1.
CONCLUSION
Our dataset is useful to further elucidate the molecular mechanisms underlying decidualization.
目的
蜕膜化对于胚胎着床和胎盘发育至关重要。本研究旨在获得原代子宫内膜基质细胞（ESCs）和体外蜕膜化细胞的转录组和表观基因组图谱。
材料与方法
从人子宫内膜组织中分离出的 ESCs 在存在 8-溴-cAMP 和孕酮的情况下未处理（D0）或蜕膜化 4 天（D4）和 8 天（D8）。
结果
在所检查的表观遗传修饰（DNA 甲基化、H3K27ac、H3K9me3 和 H3K27me3）中，H3K27ac 模式在蜕膜化过程中变化最显著，与基因表达变化呈中度相关。蜕膜化时上调和下调基因的亚群与启动子区域 H3K27ac 和 H3K27me3 修饰的相互变化相关，并富集了 WNT4、ZBTB16、PROK1 和 GREB1 等对蜕膜化至关重要的基因。
结论
我们的数据集可用于进一步阐明蜕膜化的分子机制。
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