Miluchová Martina, Gábor Michal, Candrák Juraj, Trakovická Anna, Candráková Kristína
Department of Genetics and Breeding Biology, Slovak University of Agriculture in Nitra, Nitra, Slovakia.
Acta Biochim Pol. 2018;65(3):403-407. doi: 10.18388/abp.2017_2313. Epub 2018 Sep 15.
The aim of this paper was to evaluate the effect of genetic polymorphism of kappa-casein on milk production in Holstein cattle. Two hundred and ten Holstein cows were used in this study. We established genotype structure of cattle population and calculated allelic frequencies based on PCR-RFLP analyses. The three genotypes: AA (69.52%), AB (27.62%), and BB (2.86%) were detected. Frequency of allele A was 83.33%, and of allele B 16.67%. The Holstein cattle kept in Slovak Republic exhibit a high value of homozygosity (0.7222) and low values of polymorphism information content (0.2392), effective number of alleles (1.3847) and level of possible variability realization (27.91%). The effect of polymorphism of CSN3 gene on average breeding values for milk production traits, such as yield of milk, fat and protein expressed in kilograms, as well as percentage content of fat and protein in milk, has been assessed. In our assessment of the observed traits' variability's dependence on CSN3 gene polymorphism, we detected a statistically significant difference between genotypes only in case of the average breeding value for the percentage of protein in milk.
本文旨在评估κ-酪蛋白基因多态性对荷斯坦奶牛产奶量的影响。本研究使用了210头荷斯坦奶牛。我们基于PCR-RFLP分析建立了牛群的基因型结构并计算了等位基因频率。检测到三种基因型：AA（69.52%）、AB（27.62%）和BB（2.86%）。等位基因A的频率为83.33%，等位基因B的频率为16.67%。饲养在斯洛伐克共和国的荷斯坦奶牛表现出较高的纯合度值（0.7222）以及较低的多态性信息含量值（0.2392）、有效等位基因数（1.3847）和可能变异实现水平（27.91%）。已评估了CSN3基因多态性对产奶性状平均育种值的影响，这些性状包括以千克表示的牛奶、脂肪和蛋白质产量，以及牛奶中脂肪和蛋白质的百分比含量。在我们评估观察到的性状变异性对CSN3基因多态性的依赖性时，仅在牛奶中蛋白质百分比的平均育种值方面检测到基因型之间存在统计学上的显著差异。
