脊髓灰质炎病毒RNA中重组的优先位点：对40个型间交叉序列的分析
Preferred sites of recombination in poliovirus RNA: an analysis of 40 intertypic cross-over sequences.
作者信息
King A M
机构信息
AFRC Institute for Animal Health, Pirbright Laboratory, Surrey, UK.
出版信息
Nucleic Acids Res. 1988 Dec 23;16(24):11705-23. doi: 10.1093/nar/16.24.11705.
Abstract
The genome of poliovirus consists of a single strand of RNA approximately 7.5 kb long. Analysis of the sequences around 40 unique recombination sites reveals several features that differ significantly from those expected of randomly located sites. These features, which include a broad zone of elevated homology on the 3' side of the cross-over, support the theory that RNA recombination occurs by a template-switching mechanism during synthesis of the complementary strand, and that sites are chosen to minimise the adverse free energy change involved in switching to a heterotypic template. There is also a strong sequence bias, almost two-thirds of cross-overs, according to a computer simulation, occurring immediately after synthesis of UU. These features shed new light on the extent of base-pairing in replicative intermediate RNA, and on the mechanism of chain initiation.
摘要
脊髓灰质炎病毒的基因组由一条约7.5 kb长的单链RNA组成。对40个独特重组位点周围序列的分析揭示了几个与随机定位位点预期特征显著不同的特征。这些特征包括在交叉点3'侧有一个广泛的同源性升高区域，支持了RNA重组是在互补链合成过程中通过模板转换机制发生的理论，并且选择位点是为了最小化切换到异型模板所涉及的不利自由能变化。根据计算机模拟，还存在强烈的序列偏向性，近三分之二的交叉发生在UU合成之后。这些特征为复制中间RNA中的碱基配对程度以及链起始机制提供了新的线索。
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本文引用的文献
1
EVIDENCE OF GENETIC RECOMBINATION IN FOOT-AND-MOUTH DISEASE VIRUS.口蹄疫病毒基因重组的证据
Virology. 1965 Jan;25:48-54. doi: 10.1016/0042-6822(65)90250-3.
2
Genetic recombination with Newcastle disease virus, polioviruses, and influenza.与新城疫病毒、脊髓灰质炎病毒和流感病毒的基因重组。
Cold Spring Harb Symp Quant Biol. 1962;27:303-9. doi: 10.1101/sqb.1962.027.001.028.
3
Genetic recombination with poliovirus type 1. Studies of crosses between a normal horse serum-resistant mutant and several guanidine-resistant mutants of the same strain.与1型脊髓灰质炎病毒的基因重组。同一毒株的正常抗马血清突变体与几种抗胍突变体之间杂交的研究。
Virology. 1963 May;20:107-19. doi: 10.1016/0042-6822(63)90145-4.
4
Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon 12a1b.神经毒性脊髓灰质炎病毒P3/Leon/37及其减毒的萨宾疫苗衍生物P3/Leon 12a1b的基因组完整核苷酸序列比较。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1539-43. doi: 10.1073/pnas.81.5.1539.
5
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.减毒脊髓灰质炎病毒萨宾1型毒株基因组的完整核苷酸序列。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5793-7. doi: 10.1073/pnas.79.19.5793.
6
The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1.脊髓灰质炎病毒3型leon 12 a1b的核苷酸序列：与脊髓灰质炎病毒1型的比较。
Nucleic Acids Res. 1983 Aug 25;11(16):5629-43. doi: 10.1093/nar/11.16.5629.
7
Primary structure, gene organization and polypeptide expression of poliovirus RNA.脊髓灰质炎病毒RNA的一级结构、基因组织及多肽表达
Nature. 1981 Jun 18;291(5816):547-53. doi: 10.1038/291547a0.
8
Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants.所有三种脊髓灰质炎病毒血清型基因组的完整核苷酸序列。对遗传关系、基因功能和抗原决定簇的意义。
J Mol Biol. 1984 Apr 25;174(4):561-85. doi: 10.1016/0022-2836(84)90084-6.
9
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J Mol Biol. 1984 Mar 5;173(3):325-40. doi: 10.1016/0022-2836(84)90124-4.
10
A genetic map of poliovirus temperature-sensitive mutants.脊髓灰质炎病毒温度敏感突变体的遗传图谱。
Virology. 1968 Aug;35(4):584-96. doi: 10.1016/0042-6822(68)90287-0.
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