Mallis Panagiotis, Michalopoulos Efstathios, Dinou Amalia, Vlachou Maria Spyropoulou, Panagouli Efrosyni, Papapanagiotou Aggeliki, Kassi Eva, Giokas Catherine Stavropoulos
Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, Greece; Department of Biological Chemistry, Medical School, National and Kapodistrian Univesity of Athens, Greece.
Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, Greece.
Hum Immunol. 2018 Dec;79(12):855-860. doi: 10.1016/j.humimm.2018.09.001. Epub 2018 Sep 11.
Worldwide, there is a great need of small diameter vascular grafts that can be used in human disorders such as cardiovascular and peripheral vascular disease. Until now, severe adverse reactions are caused from the use of synthetic or animal derived grafts, while the use of autologous vessels is restricted only in a small number of patients. The limited availability of the vessels might be resolved by the use of HLA-matched vascular grafts utilizing the decellularized human umbilical arteries. In this study, human umbilical arteries were decellularized and then repopulated with Mesenchymal Stem Cells. The HLA-genotype of the repopulated grafts, analyzed by Next Generation Sequencing technology, indicated their successful production. The HLA-matched vascular grafts could be generated efficiently and might be used in personalized medicine.
在全球范围内，对于可用于治疗诸如心血管疾病和外周血管疾病等人类疾病的小直径血管移植物有巨大需求。到目前为止，使用合成或动物源移植物会引发严重不良反应，而自体血管的使用仅局限于少数患者。利用脱细胞人脐动脉的HLA匹配血管移植物或许可以解决血管供应有限的问题。在本研究中，人脐动脉被脱细胞处理，然后再植入间充质干细胞。通过下一代测序技术分析再植入移植物的HLA基因型，表明它们已成功制备。HLA匹配的血管移植物能够高效生成，并且可能用于个性化医疗。
