Hammad Mira, Dugué Justin, Maubert Eric, Baugé Catherine, Boumédiene Karim
Laboratoire BioConnect UR 7451, Université de Caen Normandie, Esplanade de la paix CS14032, Caen, 14032 Caen Cedex 5, France.
Fédération Hospitalo Universitaire SURFACE, Amiens, Caen, Rouen, France.
J Biol Eng. 2025 Apr 22;19(1):38. doi: 10.1186/s13036-025-00502-2.
Decellularized plant tissues have been shown to enhance the integration and proliferation of human cells, demonstrating biocompatibility. These tissues are now being considered as valuable biomaterials for tissue engineering due to their diverse architectures and favorable cytocompatibility. In this study, we assessed decellularized apple hypanthium as a potential biomaterial for generating cartilage-like structures, utilizing four different progenitor cell types and varying environmental conditions in vitro.
Cell viability assays indicated integration and cell proliferation. Histological staining and gene expression analyses confirmed the synthesis and deposition of a cartilaginous extracellular matrix. Notably, hypoxia had varying effects on chondrogenesis based on the cell type. Among the progenitor cells evaluated, those derived from auricular perichondrium were particularly promising, as they differentiated into chondrocytes without requiring a low-oxygen environment. Additionally, our findings demonstrated that apple-derived biomaterials outperformed microencapsulation in alginate beads in promoting chondrogenesis.
These results highlight the potential of plant-based biomaterials for the development of implantable devices for cartilage regeneration and suggest broader applications in tissue engineering and future clinical endeavors.
已证明去细胞化的植物组织可增强人类细胞的整合与增殖，显示出生物相容性。由于其多样的结构和良好的细胞相容性，这些组织现在被视为组织工程中有价值的生物材料。在本研究中，我们评估了去细胞化的苹果花托作为生成类软骨结构的潜在生物材料，在体外利用四种不同的祖细胞类型和不同的环境条件。
细胞活力测定表明细胞整合和增殖。组织学染色和基因表达分析证实了软骨细胞外基质的合成与沉积。值得注意的是，缺氧根据细胞类型对软骨形成有不同影响。在评估的祖细胞中，源自耳廓软骨膜的祖细胞特别有前景，因为它们无需低氧环境就能分化为软骨细胞。此外，我们的研究结果表明，苹果衍生的生物材料在促进软骨形成方面优于藻酸盐珠中的微囊化。
这些结果突出了植物基生物材料在开发用于软骨再生的可植入装置方面的潜力，并表明其在组织工程和未来临床应用中的更广泛应用。
